Premature Lambs Exhibit Normal Mitochondrial Respiration after Long-Term Extrauterine Support.
8-Hydroxy-2'-Deoxyguanosine
/ blood
Animals
Animals, Newborn
Artificial Organs
Bilirubin
/ blood
Biomarkers
/ blood
Cell Respiration
Energy Metabolism
Extracorporeal Membrane Oxygenation
/ instrumentation
Female
Fetal Monitoring
Gestational Age
Mitochondria
/ metabolism
Oxygen Consumption
Oxygenators, Membrane
Pregnancy
Premature Birth
/ metabolism
Sheep, Domestic
Time Factors
Artificial womb
Extrauterine support
Fetus
Mitochondria
Oxygen consumption
Respiration
Journal
Fetal diagnosis and therapy
ISSN: 1421-9964
Titre abrégé: Fetal Diagn Ther
Pays: Switzerland
ID NLM: 9107463
Informations de publication
Date de publication:
2019
2019
Historique:
received:
28
09
2018
accepted:
13
12
2018
pubmed:
13
3
2019
medline:
9
4
2020
entrez:
13
3
2019
Statut:
ppublish
Résumé
In an effort to mitigate the major morbidities and mortality associated with extreme prematurity, we have developed an EXTrauterine Environment for Neonatal Development (EXTEND) designed to provide physiologic support of extremely premature infants. We have previously shown that long-term, physiologic support of premature fetal lambs is possible with EXTEND, but in this study, we sought to demonstrate bioenergetic equipoise at the tissue level. Four premature fetal lambs were delivered by hysterotomy at gestational ages (GA) of 105-107 days (term ∼145 days), cannulated via the umbilical vessels, and transitioned to support on EXTEND for 3-4 weeks. Five control fetuses were age-matched to the GA of experimental fetuses at the time of study end (128-134 days GA) and immediately sacrificed after hysterotomy. Mitochondria were isolated from the heart, liver, kidney, and skeletal muscle of fetuses at the time of sacrifice, and oxygen consumption rates (OCRs) were measured. There were no differences in basal mitochondrial OCR between EXTEND and control fetuses for heart, kidney, or skeletal muscle. For liver, the basal OCR was higher in EXTEND fetuses compared to controls. There were no differences in physiologic maximal OCR or reserve capacity for any tissue analyzed. Fetal lambs supported by EXTEND demonstrate physiologic mitochondrial function as evidenced by adequate basal and physiologic maximal cellular respiration as well as preserved reserve capacity.
Sections du résumé
BACKGROUND
BACKGROUND
In an effort to mitigate the major morbidities and mortality associated with extreme prematurity, we have developed an EXTrauterine Environment for Neonatal Development (EXTEND) designed to provide physiologic support of extremely premature infants.
OBJECTIVES
OBJECTIVE
We have previously shown that long-term, physiologic support of premature fetal lambs is possible with EXTEND, but in this study, we sought to demonstrate bioenergetic equipoise at the tissue level.
METHODS
METHODS
Four premature fetal lambs were delivered by hysterotomy at gestational ages (GA) of 105-107 days (term ∼145 days), cannulated via the umbilical vessels, and transitioned to support on EXTEND for 3-4 weeks. Five control fetuses were age-matched to the GA of experimental fetuses at the time of study end (128-134 days GA) and immediately sacrificed after hysterotomy. Mitochondria were isolated from the heart, liver, kidney, and skeletal muscle of fetuses at the time of sacrifice, and oxygen consumption rates (OCRs) were measured.
RESULTS
RESULTS
There were no differences in basal mitochondrial OCR between EXTEND and control fetuses for heart, kidney, or skeletal muscle. For liver, the basal OCR was higher in EXTEND fetuses compared to controls. There were no differences in physiologic maximal OCR or reserve capacity for any tissue analyzed.
CONCLUSIONS
CONCLUSIONS
Fetal lambs supported by EXTEND demonstrate physiologic mitochondrial function as evidenced by adequate basal and physiologic maximal cellular respiration as well as preserved reserve capacity.
Identifiants
pubmed: 30861524
pii: 000496232
doi: 10.1159/000496232
doi:
Substances chimiques
Biomarkers
0
8-Hydroxy-2'-Deoxyguanosine
88847-89-6
Bilirubin
RFM9X3LJ49
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
306-312Informations de copyright
© 2019 S. Karger AG, Basel.