Highly Multiplexed Fluorescence in Situ Hybridization for in Situ Genomics.
Journal
The Journal of molecular diagnostics : JMD
ISSN: 1943-7811
Titre abrégé: J Mol Diagn
Pays: United States
ID NLM: 100893612
Informations de publication
Date de publication:
05 2019
05 2019
Historique:
received:
29
01
2018
revised:
16
12
2018
accepted:
30
01
2019
pubmed:
14
3
2019
medline:
19
6
2020
entrez:
14
3
2019
Statut:
ppublish
Résumé
The quantification of changes in gene copy number is critical to our understanding of tumor biology and for the clinical management of cancer patients. DNA fluorescence in situ hybridization is the gold standard method to detect copy number alterations, but it is limited by the number of genes one can quantify simultaneously. To increase the throughput of this informative technique, a fluorescent bar-code system for the unique labeling of dozens of genes and an automated image analysis algorithm that enabled their simultaneous hybridization for the quantification of gene copy numbers were devised. We demonstrate the reliability of this multiplex approach on normal human lymphocytes, metaphase spreads of transformed cell lines, and cultured circulating tumor cells. It also opens the door to the development of gene panels for more comprehensive analysis of copy number changes in tissue, including the study of heterogeneity and of high-throughput clinical assays that could provide rapid quantification of gene copy numbers in samples with limited cellularity, such as circulating tumor cells.
Identifiants
pubmed: 30862547
pii: S1525-1578(18)30022-9
doi: 10.1016/j.jmoldx.2019.01.010
pmc: PMC6504678
pii:
doi:
Substances chimiques
Fluorescent Dyes
0
Molecular Probes
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
390-407Subventions
Organisme : NCI NIH HHS
ID : R01 CA129933
Pays : United States
Organisme : NCI NIH HHS
ID : R21 CA183686
Pays : United States
Organisme : NCRR NIH HHS
ID : S10 RR029237
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR001102
Pays : United States
Informations de copyright
Copyright © 2019 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. All rights reserved.
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