Extraction and Purification of Wall-Bound Polymers of Gram-Positive Bacteria.
Bacillus anthracis
/ chemistry
Cell Wall
/ chemistry
Chemical Fractionation
/ methods
Chromatography, Gel
/ methods
Chromatography, High Pressure Liquid
/ methods
Chromatography, Reverse-Phase
/ methods
Gram-Positive Bacteria
/ chemistry
Gram-Positive Bacterial Infections
/ microbiology
Humans
Hydrofluoric Acid
/ chemistry
Peptidoglycan
/ chemistry
Polysaccharides, Bacterial
/ chemistry
Teichoic Acids
/ chemistry
Bacillus cereus
Hydrofluoric acid
Murein sacculus
Peptidoglycan
Phosphodiester bond
Secondary cell wall polysaccharide
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
14
3
2019
pubmed:
14
3
2019
medline:
23
7
2019
Statut:
ppublish
Résumé
The envelope of gram-positive bacteria encompasses the cell wall, a rigid exoskeleton comprised of peptidoglycan that provides protection against lysis and governs bacterial cell shapes. Peptidoglycan also serves as the site of attachment for proteins and nonproteinaceous polymers that interact with the bacterial environment. Nonproteinaceous molecules include teichoic acids, capsular polysaccharides, and secondary cell wall polysaccharides (SCWP). Treatment of gram-positive bacterial cells with proteases, nucleases, and detergents results in the isolation of "murein sacculi" (i.e., peptidoglycan with bound carbohydrate polymers). Incubation of sacculi with acid or base releases carbohydrate polymers that can be purified for further biochemical characterization. This protocol describes the hydrofluoric acid extraction and purification of the secondary cell wall polymer of Bacillus anthracis that is also found in the envelope of the other members of the Bacillus cereus sensu lato group of bacteria.
Identifiants
pubmed: 30864123
doi: 10.1007/978-1-4939-9154-9_5
pmc: PMC7206762
mid: NIHMS1575932
doi:
Substances chimiques
Peptidoglycan
0
Polysaccharides, Bacterial
0
Teichoic Acids
0
Hydrofluoric Acid
RGL5YE86CZ
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
47-57Subventions
Organisme : NIAID NIH HHS
ID : R01 AI069227
Pays : United States
Organisme : NIAID NIH HHS
ID : R37 AI069227
Pays : United States
Références
J Biol Chem. 1992 Jun 5;267(16):11248-54
pubmed: 1597460
FEBS Lett. 1976 Jan 15;61(2):240-2
pubmed: 1248627
EMBO J. 2000 Sep 1;19(17):4473-84
pubmed: 10970841
J Bacteriol. 2013 Oct;195(20):4650-9
pubmed: 23935043
Microb Drug Resist. 2012 Jun;18(3):240-55
pubmed: 22432711
Glycobiology. 2012 Aug;22(8):1103-17
pubmed: 22556058
Glycobiology. 2011 Jul;21(7):934-48
pubmed: 21421577
J Bacteriol. 1985 Jan;161(1):299-306
pubmed: 3918002
J Biol Chem. 2006 Sep 22;281(38):27932-41
pubmed: 16870610
Acta Microbiol Acad Sci Hung. 1971;18(2):105-8
pubmed: 5004640
J Bacteriol. 2008 Jan;190(1):112-21
pubmed: 17981984
Biochemistry. 1967 Dec;6(12):3659-70
pubmed: 4864856
J Biol Chem. 2014 May 30;289(22):15680-90
pubmed: 24753256
J Bacteriol. 1997 Jun;179(12):3892-8
pubmed: 9190804
Biochim Biophys Acta. 2014 Aug;1843(8):1687-97
pubmed: 24269844
J Bacteriol. 2018 Feb 7;200(5):
pubmed: 29229702
Microbiol Mol Biol Rev. 1999 Mar;63(1):174-229
pubmed: 10066836
Science. 1995 Apr 7;268(5207):103-6
pubmed: 7701329
Annu Rev Microbiol. 2017 Sep 8;71:79-98
pubmed: 28622090
J Am Chem Soc. 2012 Sep 19;134(37):15556-62
pubmed: 22935003
EMBO J. 2011 Sep 30;30(24):4931-41
pubmed: 21964069
J Bacteriol. 2015 Jan;197(2):343-53
pubmed: 25384480