Biofilm Specific Activity: A Measure to Quantify Microbial Biofilm.
2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT)
Candida spp.
Staphylococcus aureus
biofilm
crystal violet
quantification
Journal
Microorganisms
ISSN: 2076-2607
Titre abrégé: Microorganisms
Pays: Switzerland
ID NLM: 101625893
Informations de publication
Date de publication:
07 Mar 2019
07 Mar 2019
Historique:
received:
04
02
2019
revised:
23
02
2019
accepted:
04
03
2019
entrez:
15
3
2019
pubmed:
15
3
2019
medline:
15
3
2019
Statut:
epublish
Résumé
Microbes growing onto solid surfaces form complex 3-D biofilm structures characterized by the production of extracellular polymeric compounds and an increased resistance to drugs. The quantification of biofilm relays currently on a number of different approaches and techniques, often leading to different evaluations of the ability to form biofilms of the studied microbial strains. Measures of biofilm biomass were carried out with crystal violet (CV) and a direct reading at 405 nm, whereas the activity was assessed with the XTT ((2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2
Identifiants
pubmed: 30866438
pii: microorganisms7030073
doi: 10.3390/microorganisms7030073
pmc: PMC6463164
pii:
doi:
Types de publication
Journal Article
Langues
eng
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