A Hox-TALE regulatory circuit for neural crest patterning is conserved across vertebrates.
Animals
Animals, Genetically Modified
Binding Sites
/ genetics
Cell Line
Conserved Sequence
/ physiology
Enhancer Elements, Genetic
/ genetics
Gene Expression Regulation, Developmental
/ physiology
Genes, Homeobox
/ physiology
Homeodomain Proteins
/ genetics
Lampreys
Mice
Mouse Embryonic Stem Cells
Neural Crest
/ embryology
Sequence Alignment
Vertebrates
/ embryology
Zebrafish
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
13 03 2019
13 03 2019
Historique:
received:
05
12
2018
accepted:
26
02
2019
entrez:
15
3
2019
pubmed:
15
3
2019
medline:
6
4
2019
Statut:
epublish
Résumé
In jawed vertebrates (gnathostomes), Hox genes play an important role in patterning head and jaw formation, but mechanisms coupling Hox genes to neural crest (NC) are unknown. Here we use cross-species regulatory comparisons between gnathostomes and lamprey, a jawless extant vertebrate, to investigate conserved ancestral mechanisms regulating Hox2 genes in NC. Gnathostome Hoxa2 and Hoxb2 NC enhancers mediate equivalent NC expression in lamprey and gnathostomes, revealing ancient conservation of Hox upstream regulatory components in NC. In characterizing a lamprey hoxα2 NC/hindbrain enhancer, we identify essential Meis, Pbx, and Hox binding sites that are functionally conserved within Hoxa2/Hoxb2 NC enhancers. This suggests that the lamprey hoxα2 enhancer retains ancestral activity and that Hoxa2/Hoxb2 NC enhancers are ancient paralogues, which diverged in hindbrain and NC activities. This identifies an ancestral mechanism for Hox2 NC regulation involving a Hox-TALE regulatory circuit, potentiated by inputs from Meis and Pbx proteins and Hox auto-/cross-regulatory interactions.
Identifiants
pubmed: 30867425
doi: 10.1038/s41467-019-09197-8
pii: 10.1038/s41467-019-09197-8
pmc: PMC6416258
doi:
Substances chimiques
Homeodomain Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1189Références
Trends Genet. 2017 Oct;33(10):715-727
pubmed: 28851604
Development. 2012 Jun;139(12):2091-9
pubmed: 22619386
Dev Biol. 2006 Aug 1;296(1):190-202
pubmed: 16750825
Development. 2000 Jan;127(1):155-66
pubmed: 10654609
Dev Biol. 2001 May 15;233(2):468-81
pubmed: 11336508
Wiley Interdiscip Rev Dev Biol. 2017 Nov;6(6):
pubmed: 28771970
Genes Dev. 1997 Jul 15;11(14):1885-95
pubmed: 9242495
Genome Biol. 2018 Oct 17;19(1):166
pubmed: 30333059
Science. 2016 Jan 29;351(6272):aad2197
pubmed: 26823433
Nat Biotechnol. 2005 Aug;23(8):988-94
pubmed: 16041365
Dev Biol. 1999 Sep 1;213(1):131-41
pubmed: 10452851
Nat Cell Biol. 2000 Feb;2(2):96-102
pubmed: 10655589
Genome Res. 2008 Jul;18(7):1127-32
pubmed: 18562679
Cell. 2007 Apr 20;129(2):277-88
pubmed: 17448990
Genome Biol. 2018 Nov 28;19(1):209
pubmed: 30486862
Nature. 2014 Oct 23;514(7523):490-3
pubmed: 25219855
Dev Biol. 2016 Jan 15;409(2):530-42
pubmed: 26632170
Proc Natl Acad Sci U S A. 2017 Jun 6;114(23):5838-5845
pubmed: 28584089
Genesis. 2006 Jan;44(1):23-8
pubmed: 16400644
Nature. 1994 Aug 18;370(6490):563-6
pubmed: 7914353
Nature. 2004 Sep 2;431(7004):67-71
pubmed: 15343333
Dev Biol. 2008 Dec 1;324(1):152-60
pubmed: 18801357
Development. 2002 Feb;129(3):585-95
pubmed: 11830560
Nucleic Acids Res. 1997 Nov 1;25(21):4173-80
pubmed: 9336443
Genome Res. 2017 Sep;27(9):1501-1512
pubmed: 28784834
Nucleic Acids Res. 2008 Jun;36(10):3214-25
pubmed: 18417536
Dev Biol. 2006 Mar 15;291(2):193-206
pubmed: 16515781
Nature. 2004 May 20;429(6989):1 p following 262
pubmed: 15154395
Nucleic Acids Res. 2018 Jan 4;46(D1):D1284
pubmed: 29161433
Cell. 1993 Jan 29;72(2):183-96
pubmed: 8093858
Nucleic Acids Res. 2012 May;40(9):3990-4001
pubmed: 22223247
Dev Biol. 2018 Dec 1;444 Suppl 1:S67-S78
pubmed: 29571614
Dev Biol. 2002 Jun 1;246(1):45-56
pubmed: 12027433
J Anat. 2013 Jan;222(1):19-31
pubmed: 22414251
Nat Genet. 2018 Feb;50(2):270-277
pubmed: 29358652
Development. 2010 Aug;137(16):2605-21
pubmed: 20663816
Curr Protoc Mol Biol. 2015 Jan 05;109:21.29.1-21.29.9
pubmed: 25559105
Development. 1996 Feb;122(2):543-54
pubmed: 8625806
Dev Biol. 2015 Jun 15;402(2):162-74
pubmed: 25889273
Cold Spring Harb Protoc. 2009 Jan;2009(1):pdb.emo113
pubmed: 20147008
PLoS Biol. 2017 Apr 19;15(4):e2001573
pubmed: 28422959
Nat Ecol Evol. 2018 May;2(5):859-866
pubmed: 29610468
Mol Cell Biol. 1997 Oct;17(10):5679-87
pubmed: 9315626
Zoological Lett. 2017 Sep 15;3:17
pubmed: 28932414
Development. 1999 Apr;126(7):1483-94
pubmed: 10068641
Dev Biol. 2001 Mar 1;231(1):201-16
pubmed: 11180963
Nature. 2000 Dec 14;408(6814):854-7
pubmed: 11130723
Science. 2002 Feb 15;295(5558):1288-91
pubmed: 11847340
Nat Protoc. 2006;1(3):1297-305
pubmed: 17406414
BMC Dev Biol. 2015 Nov 06;15:40
pubmed: 26545946
J Exp Zool B Mol Dev Evol. 2005 Jul 15;304(4):274-97
pubmed: 16003768
Nat Methods. 2012 Mar 04;9(4):357-9
pubmed: 22388286
Proc Natl Acad Sci U S A. 2004 Oct 19;101(42):15118-23
pubmed: 15469921
Science. 2009 Jun 26;324(5935):1720-3
pubmed: 19443739
Dev Dyn. 2006 Jun;235(6):1522-31
pubmed: 16538655
Dev Cell. 2007 Sep;13(3):405-20
pubmed: 17765683
Development. 1994 Apr;120(4):911-23
pubmed: 7600967
Dev Biol. 2007 Aug 15;308(2):606-20
pubmed: 17560975
Development. 2010 May;137(9):1505-13
pubmed: 20335361
Trends Genet. 2016 Jun;32(6):334-347
pubmed: 27066866
Int J Dev Biol. 2008;52(1):9-20
pubmed: 18033668
Chem Biol. 2010 Jan 29;17(1):65-74
pubmed: 20142042
Proc Natl Acad Sci U S A. 2013 Oct 1;110(40):16044-9
pubmed: 24043829
Mol Cell Biol. 1994 Jun;14(6):3938-48
pubmed: 7910944
Nature. 2015 Apr 23;520(7548):474-482
pubmed: 25903629
Nature. 2017 Apr 6;544(7648):88-91
pubmed: 28321127
Genome Res. 2003 Apr;13(4):721-31
pubmed: 12654723
PLoS One. 2014 Jan 09;9(1):e85492
pubmed: 24416417
Dev Biol. 2017 Dec 1;432(1):151-164
pubmed: 28982536
Dev Cell. 2015 Feb 9;32(3):265-77
pubmed: 25640223
Development. 2004 Feb;131(3):613-27
pubmed: 14711874
Wiley Interdiscip Rev Dev Biol. 2013 Jan-Feb;2(1):1-15
pubmed: 23799627
Dev Biol. 2007 Feb 15;302(2):646-60
pubmed: 17113575
Dev Biol. 2010 Aug 1;344(1):358-62
pubmed: 20435033
Nature. 2004 Oct 7;431(7009):696-9
pubmed: 15470430
Genome Res. 2015 Aug;25(8):1229-43
pubmed: 26025802
Methods Cell Biol. 2004;74:171-94
pubmed: 15575607
Elife. 2014 Mar 18;3:e01939
pubmed: 24642410
Development. 2001 Aug;128(15):3017-27
pubmed: 11532923
Integr Comp Biol. 2006 Dec;46(6):890-901
pubmed: 21672793