Fixed-target serial oscillation crystallography at room temperature.

X-ray crystallography fixed-target serial oscillation oscillations radiation damage serial crystallography storage rings structural biology structure determination

Journal

IUCrJ
ISSN: 2052-2525
Titre abrégé: IUCrJ
Pays: England
ID NLM: 101623101

Informations de publication

Date de publication:
01 Mar 2019
Historique:
received: 18 11 2018
accepted: 25 01 2019
entrez: 15 3 2019
pubmed: 15 3 2019
medline: 15 3 2019
Statut: epublish

Résumé

A fixed-target approach to high-throughput room-temperature serial synchrotron crystallography with oscillation is described. Patterned silicon chips with microwells provide high crystal-loading density with an extremely high hit rate. The microfocus, undulator-fed beamline at CHESS, which has compound refractive optics and a fast-framing detector, was built and optimized for this experiment. The high-throughput oscillation method described here collects 1-5° of data per crystal at room temperature with fast (10° s

Identifiants

pubmed: 30867928
doi: 10.1107/S2052252519001453
pii: mf5030
pmc: PMC6400179
doi:

Types de publication

Journal Article

Langues

eng

Pagination

305-316

Subventions

Organisme : NIGMS NIH HHS
ID : P41 GM103485
Pays : United States

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Auteurs

Jennifer L Wierman (JL)

MacCHESS, Cornell University, Ithaca, NY 14853, USA.

Olivier Paré-Labrosse (O)

Departments of Chemistry and Physics, University of Toronto, Toronto, ON Canada.
Max Planck Institute for the Structure and Dynamics of Matter, Hamburg, Germany.

Antoine Sarracini (A)

Departments of Chemistry and Physics, University of Toronto, Toronto, ON Canada.

Jessica E Besaw (JE)

Departments of Chemistry and Physics, University of Toronto, Toronto, ON Canada.

Michael J Cook (MJ)

MacCHESS, Cornell University, Ithaca, NY 14853, USA.

Saeed Oghbaey (S)

Departments of Chemistry and Physics, University of Toronto, Toronto, ON Canada.

Hazem Daoud (H)

Departments of Chemistry and Physics, University of Toronto, Toronto, ON Canada.

Pedram Mehrabi (P)

Max Planck Institute for the Structure and Dynamics of Matter, Hamburg, Germany.

Irina Kriksunov (I)

MacCHESS, Cornell University, Ithaca, NY 14853, USA.

Anling Kuo (A)

Departments of Biochemistry and Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.

David J Schuller (DJ)

MacCHESS, Cornell University, Ithaca, NY 14853, USA.

Scott Smith (S)

MacCHESS, Cornell University, Ithaca, NY 14853, USA.

Oliver P Ernst (OP)

Departments of Biochemistry and Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.

Doletha M E Szebenyi (DME)

MacCHESS, Cornell University, Ithaca, NY 14853, USA.

Sol M Gruner (SM)

MacCHESS, Cornell University, Ithaca, NY 14853, USA.
Department of Physics, Cornell University, Ithaca, NY 14853, USA.
Kavli Institute for Nanoscale Science, Cornell University, Ithaca, NY 14853, USA.

R J Dwayne Miller (RJD)

Departments of Chemistry and Physics, University of Toronto, Toronto, ON Canada.
Max Planck Institute for the Structure and Dynamics of Matter, Hamburg, Germany.

Aaron D Finke (AD)

MacCHESS, Cornell University, Ithaca, NY 14853, USA.

Classifications MeSH