Bacteriophages Isolated in China for the Control of Pectobacterium carotovorum Causing Potato Soft Rot in Kenya.
Bacteriophages
Pectobacterium carotovorum
Phage resistance
Potato soft rot
Journal
Virologica Sinica
ISSN: 1995-820X
Titre abrégé: Virol Sin
Pays: Netherlands
ID NLM: 101514185
Informations de publication
Date de publication:
Jun 2019
Jun 2019
Historique:
received:
03
08
2018
accepted:
10
12
2018
pubmed:
15
3
2019
medline:
14
8
2019
entrez:
15
3
2019
Statut:
ppublish
Résumé
Soft rot is an economically significant disease in potato and one of the major threats to sustainable potato production. This study aimed at isolating lytic bacteriophages and evaluating methods for and the efficacy of applying phages to control potato soft rot caused by Pectobacterium carotovorum. Eleven bacteriophages isolated from soil and water samples collected in Wuhan, China, were used to infect P. carotovorum host strains isolated from potato tubers showing soft rot symptoms in Nakuru county, Kenya. The efficacy of the phages in controlling soft rot disease was evaluated by applying individual phage strains or a phage cocktail on potato slices and tubers at different time points before or after inoculation with a P. carotovorum strain. The phages could lyse 20 strains of P. carotovorum, but not Pseudomonas fluorescens control strains. Among the 11 phages, Pectobacterium phage Wc5r, interestingly showed cross-activity against Pectobacterium atrosepticum and two phage-resistant P. carotovorum strains. Potato slice assays showed that the phage concentration and timing of application are crucial factors for effective soft rot control. Phage cocktail applied at a concentration of 1 × 10
Identifiants
pubmed: 30868359
doi: 10.1007/s12250-019-00091-7
pii: 10.1007/s12250-019-00091-7
pmc: PMC6599505
doi:
Substances chimiques
Biological Control Agents
0
Types de publication
Journal Article
Langues
eng
Pagination
287-294Références
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