CXCL4/PF4 is a predictive biomarker of cardiac differentiation potential of human induced pluripotent stem cells.
Biomarkers
/ metabolism
Cell Differentiation
/ physiology
Cells, Cultured
Cellular Reprogramming
/ physiology
Humans
Induced Pluripotent Stem Cells
/ cytology
MicroRNAs
/ genetics
Myocytes, Cardiac
/ metabolism
Organogenesis
/ physiology
Platelet Factor 4
/ genetics
RNA, Messenger
/ genetics
Transcriptome
/ genetics
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
15 03 2019
15 03 2019
Historique:
received:
31
08
2018
accepted:
21
02
2019
entrez:
16
3
2019
pubmed:
16
3
2019
medline:
2
10
2020
Statut:
epublish
Résumé
Selection of human induced pluripotent stem cell (hiPSC) lines with high cardiac differentiation potential is important for regenerative therapy and drug screening. We aimed to identify biomarkers for predicting cardiac differentiation potential of hiPSC lines by comparing the gene expression profiles of six undifferentiated hiPSC lines with different cardiac differentiation capabilities. We used three platforms of gene expression analysis, namely, cap analysis of gene expression (CAGE), mRNA array, and microRNA array to efficiently screen biomarkers related to cardiac differentiation of hiPSCs. Statistical analysis revealed candidate biomarker genes with significant correlation between the gene expression levels in the undifferentiated hiPSCs and their cardiac differentiation potential. Of the candidate genes, PF4 was validated as a biomarker expressed in undifferentiated hiPSCs with high potential for cardiac differentiation in 13 additional hiPSC lines. Our observations suggest that PF4 may be a useful biomarker for selecting hiPSC lines appropriate for the generation of cardiomyocytes.
Identifiants
pubmed: 30874579
doi: 10.1038/s41598-019-40915-w
pii: 10.1038/s41598-019-40915-w
pmc: PMC6420577
doi:
Substances chimiques
Biomarkers
0
MicroRNAs
0
PF4 protein, human
0
RNA, Messenger
0
Platelet Factor 4
37270-94-3
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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