Critical role of the finger loop in arrestin binding to the receptors.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
09
10
2018
accepted:
28
02
2019
entrez:
16
3
2019
pubmed:
16
3
2019
medline:
18
12
2019
Statut:
epublish
Résumé
We tested the interactions with four different G protein-coupled receptors (GPCRs) of arrestin-3 mutants with substitutions in the four loops, three of which contact the receptor in the structure of the arrestin-1-rhodopsin complex. Point mutations in the loop at the distal tip of the N-domain (Glu157Ala), in the C-loop (Phe255Ala), back loop (Lys313Ala), and one of the mutations in the finger loop (Gly65Pro) had mild variable effects on receptor binding. In contrast, the deletion of Gly65 at the beginning of the finger loop reduced the binding to all GPCRs tested, with the binding to dopamine D2 receptor being affected most dramatically. Thus, the presence of a glycine at the beginning of the finger loop appears to be critical for the arrestin-receptor interaction.
Identifiants
pubmed: 30875392
doi: 10.1371/journal.pone.0213792
pii: PONE-D-18-29351
pmc: PMC6420155
doi:
Substances chimiques
Arrestins
0
DRD1 protein, human
0
DRD2 protein, human
0
Receptor, Muscarinic M2
0
Receptors, Adrenergic, beta-2
0
Receptors, Dopamine D1
0
Receptors, Dopamine D2
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0213792Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
J Biol Chem. 1997 Sep 19;272(38):23682-9
pubmed: 9295310
J Biol Chem. 2012 Aug 24;287(35):29495-505
pubmed: 22787152
J Biol Chem. 1992 Sep 5;267(25):17882-90
pubmed: 1517224
Cell Signal. 2014 Jul;26(7):1523-31
pubmed: 24686081
Nat Methods. 2006 Mar;3(3):165-74
pubmed: 16489332
J Biol Chem. 1995 Jan 13;270(2):720-31
pubmed: 7822302
Nat Commun. 2017 Nov 10;8(1):1427
pubmed: 29127291
Cell. 2016 Aug 11;166(4):907-919
pubmed: 27499021
J Biol Chem. 2000 Mar 31;275(13):9284-9
pubmed: 10734068
Proc Natl Acad Sci U S A. 2017 Mar 7;114(10):2562-2567
pubmed: 28223524
J Mol Biol. 2011 Feb 25;406(3):467-78
pubmed: 21215759
BMC Evol Biol. 2017 Jul 6;17(1):163
pubmed: 28683816
Nature. 1989 Dec 21-28;342(6252):923-6
pubmed: 2531847
Curr Biol. 2009 Apr 28;19(8):700-5
pubmed: 19361994
J Biol Chem. 1999 Mar 12;274(11):6831-4
pubmed: 10066734
J Biol Chem. 1993 Jul 25;268(21):15640-8
pubmed: 8340388
Genes Dis. 2015 Jun;2(2):108-132
pubmed: 26229975
J Biol Chem. 2012 Mar 16;287(12):9028-40
pubmed: 22275358
Methods Mol Biol. 2014;1175:153-87
pubmed: 25150870
J Biol Chem. 2012 Jun 1;287(23):19653-64
pubmed: 22523077
Proc Natl Acad Sci U S A. 2014 Feb 4;111(5):1825-30
pubmed: 24449856
J Biol Chem. 2006 Feb 10;281(6):3458-62
pubmed: 16339758
J Mol Biol. 2005 Dec 16;354(5):1069-80
pubmed: 16289201
Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1601-6
pubmed: 11171997
Protein Sci. 1994 Dec;3(12):2428-34
pubmed: 7756996
J Biol Chem. 2011 Jul 8;286(27):24288-99
pubmed: 21471193
Proc Natl Acad Sci U S A. 2013 Jan 15;110(3):942-7
pubmed: 23277586
Genome Biol. 2006;7(9):236
pubmed: 17020596
J Biol Chem. 2009 May 29;284(22):15038-51
pubmed: 19332542
J Biol Chem. 2004 Jan 9;279(2):1262-8
pubmed: 14530255
Nat Commun. 2016 Nov 09;7:13416
pubmed: 27827372
Proc Natl Acad Sci U S A. 2012 Nov 6;109(45):18407-12
pubmed: 23091036
J Biol Chem. 2000 Dec 1;275(48):37679-85
pubmed: 10969086
Nat Commun. 2014 Sep 10;5:4801
pubmed: 25205354
J Biol Chem. 2006 Apr 7;281(14):9812-23
pubmed: 16439357
J Biol Chem. 1993 Jun 5;268(16):11628-38
pubmed: 8505295
J Biol Chem. 2002 Mar 15;277(11):9043-8
pubmed: 11782458
Cell Signal. 2017 Aug;36:98-107
pubmed: 28461104
Pharmacol Ther. 2012 Jan;133(1):40-69
pubmed: 21903131
Curr Protoc Pharmacol. 2015 Sep 01;70:2.14.1-2.14.14
pubmed: 26331887
Structure. 2001 Sep;9(9):869-80
pubmed: 11566136
J Biol Chem. 2003 Mar 28;278(13):11623-32
pubmed: 12525498
J Biol Chem. 2009 Dec 4;284(49):34103-15
pubmed: 19815545
Nat Methods. 2018 Jul;15(7):512-514
pubmed: 29786090
Cell. 2017 Jul 27;170(3):457-469.e13
pubmed: 28753425
EMBO J. 1999 Apr 1;18(7):1723-9
pubmed: 10202136
Nature. 2015 Jul 30;523(7562):561-7
pubmed: 26200343
Curr Opin Cell Biol. 2014 Apr;27:1-9
pubmed: 24680424
Trends Pharmacol Sci. 2004 Feb;25(2):105-11
pubmed: 15102497
Biochemistry. 2002 Mar 12;41(10):3321-8
pubmed: 11876640
Cell. 1999 Apr 16;97(2):257-69
pubmed: 10219246
Mol Pharmacol. 2003 Jun;63(6):1256-72
pubmed: 12761335
J Mol Biol. 2007 Apr 27;368(2):375-87
pubmed: 17359998
Nat Rev Drug Discov. 2017 Dec;16(12):829-842
pubmed: 29075003
Pharmacol Ther. 2004 Dec;104(3):173-206
pubmed: 15556674
J Biol Chem. 2016 Dec 30;291(53):27147-27159
pubmed: 27852822
Nature. 1998 Feb 26;391(6670):918-21
pubmed: 9495348
Sci Rep. 2016 Jun 28;6:28686
pubmed: 27350090
Nat Rev Drug Discov. 2008 Apr;7(4):339-57
pubmed: 18382464
Front Mol Neurosci. 2018 Jun 18;11:203
pubmed: 29973866
Nature. 1989 Dec 21-28;342(6252):926-9
pubmed: 2480527
J Biol Chem. 1997 Oct 31;272(44):27497-500
pubmed: 9346876
Curr Opin Neurobiol. 1998 Jun;8(3):335-44
pubmed: 9687355
J Biol Chem. 1995 Mar 17;270(11):6010-6
pubmed: 7890732
Nat Commun. 2018 Jan 23;9(1):341
pubmed: 29362459
J Biol Chem. 2000 Dec 29;275(52):41049-57
pubmed: 11024026