Detection of Salivary miRNAs Reflecting Chronic Periodontitis: A Pilot Study.
microRNA
periodontitis
saliva
Journal
Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009
Informations de publication
Date de publication:
15 Mar 2019
15 Mar 2019
Historique:
received:
02
02
2019
revised:
07
03
2019
accepted:
13
03
2019
entrez:
17
3
2019
pubmed:
17
3
2019
medline:
2
7
2019
Statut:
epublish
Résumé
The purpose of this cross-sectional pilot study was to find salivary microRNAs (miRNAs) reflecting periodontal condition in chronic periodontitis. One hundred and twenty chronic periodontitis patients (mean age, 68.4 years) participated in the study, from whom unstimulated whole saliva was collected. A multiphase study was conducted to explore salivary miRNAs as biomarkers of periodontitis. At first, a polymerase chain reaction (PCR) array was performed to compare salivary miRNAs profiles in no and mild (no/mild) and severe periodontitis patients. Next, the relative expression of salivary miRNAs on individual samples was assessed by real-time reverse transcription-PCR. The numbers (%) of patients were 26 (21.6%, no/mild), 58 (48.3%, moderate) and 36 (30.0%, severe), respectively. Among 84 miRNAs, only the relative expression of hsa-miR-381-3p in the severe periodontitis group was significantly higher than that of the no/mild periodontitis group (
Identifiants
pubmed: 30875931
pii: molecules24061034
doi: 10.3390/molecules24061034
pmc: PMC6470766
pii:
doi:
Substances chimiques
MIRN381 microRNA, human
0
MicroRNAs
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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