Longitudinal Analysis Reveals Early Development of Three MPER-Directed Neutralizing Antibody Lineages from an HIV-1-Infected Individual.

AIDS Vaccines / immunology Amino Acid Sequence Antibodies, Neutralizing / immunology B-Lymphocytes / immunology Cell Line HEK293 Cells HIV Antibodies / immunology HIV Infections / immunology HIV-1 / immunology Humans Leukocytes, Mononuclear Longitudinal Studies
HIV envelope neutralizing antibody next-generation sequencing

Journal

Immunity
ISSN: 1097-4180
Titre abrégé: Immunity
Pays: United States
ID NLM: 9432918

Informations de publication

Date de publication:
19 03 2019
Historique:
received: 07 08 2018
revised: 13 11 2018
accepted: 13 02 2019
pubmed: 17 3 2019
medline: 21 8 2019
entrez: 17 3 2019
Statut: ppublish

Résumé

Lineage-based vaccine design is an attractive approach for eliciting broadly neutralizing antibodies (bNAbs) against HIV-1. However, most bNAb lineages studied to date have features indicative of unusual recombination and/or development. From an individual in the prospective RV217 cohort, we identified three lineages of bNAbs targeting the membrane-proximal external region (MPER) of the HIV-1 envelope. Antibodies RV217-VRC42.01, -VRC43.01, and -VRC46.01 used distinct modes of recognition and neutralized 96%, 62%, and 30%, respectively, of a 208-strain virus panel. All three lineages had modest levels of somatic hypermutation and normal antibody-loop lengths and were initiated by the founder virus MPER. The broadest lineage, VRC42, was similar to the known bNAb 4E10. A multimeric immunogen based on the founder MPER activated B cells bearing the unmutated common ancestor of VRC42, with modest maturation of early VRC42 intermediates imparting neutralization breadth. These features suggest that VRC42 may be a promising template for lineage-based vaccine design.

Identifiants

DOI: 10.1016/j.immuni.2019.02.008 PMID: 30876875 PMC: PMC6555550
pubmed: 30876875
pii: S1074-7613(19)30074-3
doi: 10.1016/j.immuni.2019.02.008
pmc: PMC6555550
mid: NIHMS1523630
pii:
doi:

Substances chimiques

AIDS Vaccines 0
Antibodies, Neutralizing 0
HIV Antibodies 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, N.I.H., Intramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Pagination

677-691.e13

Subventions

Organisme : NIAID NIH HHS
ID : R01 AI131722
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIA AI005024-17
Pays : United States
Organisme : NIAID NIH HHS
ID : P01 AI131251
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIA AI005022-17
Pays : United States
Organisme : NCI NIH HHS
ID : HHSN261200800001E
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008320
Pays : United States

Informations de copyright

Published by Elsevier Inc.

Références

Immunity. 2012 Sep 21;37(3):412-25
pubmed: 22999947
Nat Protoc. 2016 Feb;11(2):193-213
pubmed: 26741406
PLoS Pathog. 2014 Oct 16;10(10):e1004461
pubmed: 25330199
Nat Protoc. 2008;3(7):1171-9
pubmed: 18600222
Sci Immunol. 2016 Jul 29;1(1):aag0851
pubmed: 28783677
J Virol. 2005 Aug;79(16):10108-25
pubmed: 16051804
Nat Commun. 2017 Nov 21;8(1):1655
pubmed: 29162799
J Virol. 2009 Oct;83(19):10211-23
pubmed: 19640992
J Am Med Inform Assoc. 2015 Nov;22(6):1148-52
pubmed: 26112029
Mol Biol Evol. 1996 Jan;13(1):93-104
pubmed: 8583911
PLoS One. 2011;6(9):e23532
pubmed: 21980336
J Virol. 2012 Aug;86(15):8319-23
pubmed: 22623764
J Virol. 2015 Aug;89(16):8334-45
pubmed: 26041300
N Engl J Med. 2016 Jun 2;374(22):2120-30
pubmed: 27192360
Structure. 2010 Sep 8;18(9):1116-26
pubmed: 20826338
Immunity. 2016 May 17;44(5):1215-26
pubmed: 27192579
Science. 2005 Jun 24;308(5730):1906-8
pubmed: 15860590
J Virol. 2009 Jul;83(14):7337-48
pubmed: 19439467
Curr Opin Virol. 2015 Apr;11:137-47
pubmed: 25913818
Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6470-5
pubmed: 23536288
Nature. 2012 Nov 15;491(7424):406-12
pubmed: 23151583
Anal Chem. 2014 May 20;86(10):4758-66
pubmed: 24684310
Sci Immunol. 2017 Jan 27;2(7):
pubmed: 28783671
Sci Transl Med. 2018 Jul 4;10(448):
pubmed: 29973404
J Immunol Methods. 2014 Jul;409:131-46
pubmed: 24291345
Front Immunol. 2016 Sep 21;7:372
pubmed: 27708645
J Virol. 2008 Apr;82(8):3952-70
pubmed: 18256145
Methods Enzymol. 1997;276:307-26
pubmed: 27754618
Cell. 2015 Jun 4;161(6):1280-92
pubmed: 26004070
Nature. 2014 Oct 23;514(7523):455-61
pubmed: 25296255
J Virol. 2001 Nov;75(22):10892-905
pubmed: 11602729
Bioinformatics. 2010 Oct 1;26(19):2460-1
pubmed: 20709691
Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7
pubmed: 22366317
N Engl J Med. 2012 Apr 5;366(14):1275-86
pubmed: 22475592
Cell. 2016 Apr 7;165(2):449-63
pubmed: 26949186
J Virol. 2009 Feb;83(3):1240-59
pubmed: 19019969
Science. 2016 May 13;352(6287):828-33
pubmed: 27174988
Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1131-7
pubmed: 12832755
Sci Transl Med. 2017 Mar 15;9(381):
pubmed: 28298420
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
Nat Immunol. 2017 Nov 16;18(12):1274-1278
pubmed: 29144493
Cell. 2014 Jul 31;158(3):481-91
pubmed: 25065977
PLoS Pathog. 2017 Feb 22;13(2):e1006212
pubmed: 28225819
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32
pubmed: 15572765
Immunity. 2017 Nov 21;47(5):990-1003.e9
pubmed: 29166592
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21
pubmed: 20124702
Acta Crystallogr D Biol Crystallogr. 2002 Nov;58(Pt 11):1948-54
pubmed: 12393927
AIDS. 2014 Jan 14;28(2):163-9
pubmed: 24361678
PLoS One. 2016 Jun 14;11(6):e0157409
pubmed: 27299673
Proc Natl Acad Sci U S A. 2009 Dec 1;106(48):20234-9
pubmed: 19906992
PLoS Pathog. 2017 Jan 4;13(1):e1006148
pubmed: 28052137
AIDS Res Hum Retroviruses. 1994 Apr;10(4):359-69
pubmed: 7520721
Nucleic Acids Res. 2018 Jul 2;46(W1):W296-W303
pubmed: 29788355
J Virol. 2016 Nov 14;90(23):10574-10586
pubmed: 27654288
Immunity. 2018 Jan 16;48(1):133-146.e6
pubmed: 29287996
Proc Natl Acad Sci U S A. 2014 Feb 11;111(6):2259-64
pubmed: 24469811
Immunity. 2018 May 15;48(5):855-871
pubmed: 29768174
Science. 2013 Sep 13;341(6151):1199-204
pubmed: 24031012
J Exp Med. 2013 Apr 8;210(4):655-63
pubmed: 23530120
Immunity. 2013 Aug 22;39(2):245-58
pubmed: 23911655
Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):2706-11
pubmed: 14981267
J Virol. 2015 Jan;89(1):784-98
pubmed: 25355869
Immunity. 2016 Jan 19;44(1):21-31
pubmed: 26777395
J Immunol. 2007 Apr 1;178(7):4424-35
pubmed: 17372000
Proc Natl Acad Sci U S A. 2006 May 23;103(21):8060-5
pubmed: 16690741
Nat Biotechnol. 2012 May 07;30(5):423-33
pubmed: 22565972
Vaccine. 2015 Nov 27;33 Suppl 4:D4-12
pubmed: 26100921
J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674
pubmed: 19461840
Nature. 2014 May 1;509(7498):55-62
pubmed: 24590074
PLoS One. 2011;6(11):e27824
pubmed: 22140469
Cell Rep. 2018 Feb 13;22(7):1798-1809
pubmed: 29444432
J Virol. 2012 Jul;86(14):7496-507
pubmed: 22553329
Nat Med. 2018 Jun;24(6):857-867
pubmed: 29867235
Science. 2013 May 10;340(6133):751-6
pubmed: 23661761
Biochim Biophys Acta. 2009 Mar;1788(3):660-5
pubmed: 19100711
BMC Bioinformatics. 2009 Dec 15;10:421
pubmed: 20003500
Nucleic Acids Res. 2004 Mar 19;32(5):1792-7
pubmed: 15034147
Nature. 2013 Apr 25;496(7446):469-76
pubmed: 23552890
PLoS Pathog. 2018 Mar 5;14(3):e1006860
pubmed: 29505593
Immunol Rev. 2013 Jul;254(1):225-44
pubmed: 23772623
Curr Protoc Immunol. 2005 Jan;Chapter 12:Unit 12.11
pubmed: 18432938

Auteurs

Shelly J Krebs (SJ)

U.S. Military HIV Research Program, WRAIR, Silver Spring, MD, USA; Henry Jackson Foundation, Bethesda, MD, USA.

Young D Kwon (YD)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Chaim A Schramm (CA)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

William H Law (WH)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Gina Donofrio (G)

U.S. Military HIV Research Program, WRAIR, Silver Spring, MD, USA; Henry Jackson Foundation, Bethesda, MD, USA.

Kenneth H Zhou (KH)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Syna Gift (S)

U.S. Military HIV Research Program, WRAIR, Silver Spring, MD, USA; Henry Jackson Foundation, Bethesda, MD, USA.

Vincent Dussupt (V)

U.S. Military HIV Research Program, WRAIR, Silver Spring, MD, USA; Henry Jackson Foundation, Bethesda, MD, USA.

Ivelin S Georgiev (IS)

Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN, USA.

Sebastian Schätzle (S)

Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA.

Jonathan R McDaniel (JR)

Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA.

Yen-Ting Lai (YT)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Mallika Sastry (M)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Baoshan Zhang (B)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Marissa C Jarosinski (MC)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Amy Ransier (A)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Agnes L Chenine (AL)

U.S. Military HIV Research Program, WRAIR, Silver Spring, MD, USA; Henry Jackson Foundation, Bethesda, MD, USA.

Mangaiarkarasi Asokan (M)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Robert T Bailer (RT)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Meera Bose (M)

U.S. Military HIV Research Program, WRAIR, Silver Spring, MD, USA; Henry Jackson Foundation, Bethesda, MD, USA.

Alberto Cagigi (A)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Evan M Cale (EM)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Gwo-Yu Chuang (GY)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Samuel Darko (S)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Jefferson I Driscoll (JI)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Aliaksandr Druz (A)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Jason Gorman (J)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Farida Laboune (F)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Mark K Louder (MK)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Krisha McKee (K)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Letzibeth Mendez (L)

U.S. Military HIV Research Program, WRAIR, Silver Spring, MD, USA; Henry Jackson Foundation, Bethesda, MD, USA.

M Anthony Moody (MA)

Duke University School of Medicine and Center for HIV/AIDS Vaccine Immunology - Immunogen Discovery, Durham, NC, USA.

Anne Marie O'Sullivan (AM)

U.S. Military HIV Research Program, WRAIR, Silver Spring, MD, USA; Henry Jackson Foundation, Bethesda, MD, USA.

Christopher Owen (C)

U.S. Military HIV Research Program, WRAIR, Silver Spring, MD, USA; Henry Jackson Foundation, Bethesda, MD, USA.

Dongjun Peng (D)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Reda Rawi (R)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Eric Sanders-Buell (E)

U.S. Military HIV Research Program, WRAIR, Silver Spring, MD, USA; Henry Jackson Foundation, Bethesda, MD, USA.

Chen-Hsiang Shen (CH)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Andrea R Shiakolas (AR)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Tyler Stephens (T)

Electron Microscopy Laboratory, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, Maryland, USA.

Yaroslav Tsybovsky (Y)

Electron Microscopy Laboratory, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, Maryland, USA.

Courtney Tucker (C)

U.S. Military HIV Research Program, WRAIR, Silver Spring, MD, USA; Henry Jackson Foundation, Bethesda, MD, USA.

Raffaello Verardi (R)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Keyun Wang (K)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Jing Zhou (J)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Tongqing Zhou (T)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

George Georgiou (G)

Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA.

S Munir Alam (SM)

Duke University School of Medicine and Center for HIV/AIDS Vaccine Immunology - Immunogen Discovery, Durham, NC, USA.

Barton F Haynes (BF)

Duke University School of Medicine and Center for HIV/AIDS Vaccine Immunology - Immunogen Discovery, Durham, NC, USA.

Morgane Rolland (M)

U.S. Military HIV Research Program, WRAIR, Silver Spring, MD, USA; Henry Jackson Foundation, Bethesda, MD, USA.

Gary R Matyas (GR)

U.S. Military HIV Research Program, WRAIR, Silver Spring, MD, USA.

Victoria R Polonis (VR)

U.S. Military HIV Research Program, WRAIR, Silver Spring, MD, USA.

Adrian B McDermott (AB)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Daniel C Douek (DC)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Lawrence Shapiro (L)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA; Columbia University, New York, NY, USA.

Sodsai Tovanabutra (S)

U.S. Military HIV Research Program, WRAIR, Silver Spring, MD, USA; Henry Jackson Foundation, Bethesda, MD, USA.

Nelson L Michael (NL)

U.S. Military HIV Research Program, WRAIR, Silver Spring, MD, USA.

John R Mascola (JR)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Merlin L Robb (ML)

U.S. Military HIV Research Program, WRAIR, Silver Spring, MD, USA; Henry Jackson Foundation, Bethesda, MD, USA.

Peter D Kwong (PD)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA; Columbia University, New York, NY, USA. Electronic address: pdkwong@nih.gov.

Nicole A Doria-Rose (NA)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA. Electronic address: nicole.doriarose@nih.gov.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female

Evaluation of Low-Value Services Across Major Medicare Advantage Insurers and Traditional Medicare.

Ciara Duggan, Adam L Beckman, Ishani Ganguli et al.
1.00
Humans United States Aged Cross-Sectional Studies Medicare Part C

Effectiveness of Virtual Yoga for Chronic Low Back Pain: A Randomized Clinical Trial.

Hallie Tankha, Devyn Gaskins, Amanda Shallcross et al.
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH

questionsmedicales.fr › Mélanges complexes › Produits biologiques › Vaccins › Vaccins antiviraux › Vaccins contre le SIDA › Longitudinal Analysis Reveals Early...
questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence d'acides aminés › Longitudinal Analysis Reveals Early...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps neutralisants › Longitudinal Analysis Reveals Early...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Lymphocytes › Lymphocytes B › Longitudinal Analysis Reveals Early...
questionsmedicales.fr › Cellules › Cellules cultivées › Lignée cellulaire › Longitudinal Analysis Reveals Early...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HEK293 › Longitudinal Analysis Reveals Early...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps antiviraux › Anticorps antideltarétrovirus › Anticorps anti-VIH › Longitudinal Analysis Reveals Early...
questionsmedicales.fr › Maladies du système immunitaire › Déficits immunitaires › Infections à VIH › Longitudinal Analysis Reveals Early...
questionsmedicales.fr › Virus › Virus à ARN › Retroviridae › Lentivirus › Lentivirus des primates › VIH (Virus de l'Immunodéficience Humaine) › VIH-1 (Virus de l'Immunodéficience Humaine de type 1) › Longitudinal Analysis Reveals Early...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Longitudinal Analysis Reveals Early...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Longitudinal Analysis Reveals Early...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Caractéristiques des études épidémiologiques › Études épidémiologiques › Études de cohortes › Études longitudinales › Longitudinal Analysis Reveals Early...