In silico prediction of short hairpin RNA and in vitro silencing of activin receptor type IIB in chicken embryo fibroblasts by RNA interference.
ACTRIIB
Gene expression
Gene silencing
Muscle growth
Secondary structures
Thermodynamic parameters
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
Jun 2019
Jun 2019
Historique:
received:
16
01
2019
accepted:
08
03
2019
pubmed:
18
3
2019
medline:
18
12
2019
entrez:
18
3
2019
Statut:
ppublish
Résumé
Gene silencing by RNA interference is extensively used reverse genetic approach to analyse the implications of any gene in mammalian systems. The silencing of the Activin type IIB receptor belonging to transforming growth factor beta superfamily has demonstrated increase in muscle growth in many species. We designed five short hairpin RNA constructs targeting coding region of chicken ACTRIIB. All the shRNAs were transfected into chicken embryo fibroblast cells and evaluated their silencing efficiency by real time PCR and western blotting. Initially the computational analysis of target region and shRNA constructs was undertaken to predict sequence based features (secondary structures, GC% and H-b index) and thermodynamic features (ΔG
Identifiants
pubmed: 30879273
doi: 10.1007/s11033-019-04756-0
pii: 10.1007/s11033-019-04756-0
doi:
Substances chimiques
RNA, Messenger
0
RNA, Small Interfering
0
Activin Receptors, Type II
EC 2.7.11.30
activin receptor type II-B
EC 2.7.11.30
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2947-2959Références
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