miR-146a is deregulated in gastric cancer.
Adult
Aged
Carcinogenesis
/ genetics
Disease Progression
Female
Gastric Mucosa
/ microbiology
Gene Expression Regulation, Neoplastic
Helicobacter Infections
/ microbiology
Helicobacter pylori
/ isolation & purification
Humans
Lymphatic Metastasis
/ pathology
Male
MicroRNAs
/ metabolism
Middle Aged
Neoplasm Staging
Stomach Neoplasms
/ genetics
Up-Regulation
Gastric cancer
Helicobacter pylori
miR-146a
reverse transcription quantitative polymerase chain reaction
Journal
Journal of cancer research and therapeutics
ISSN: 1998-4138
Titre abrégé: J Cancer Res Ther
Pays: India
ID NLM: 101249598
Informations de publication
Date de publication:
Historique:
entrez:
19
3
2019
pubmed:
19
3
2019
medline:
30
7
2019
Statut:
ppublish
Résumé
Gastric cancer is one of the most significant reasons for cancer-related death. miR-146a is one of the dysregulated factors associated with gastric tumorigenesis. However, deregulation of this microRNA (miRNA) has become controversial. Moreover, the inflammation-mediating role of this miRNA implies that miR-146a might be dysregulated by gastric cancer-related pathogens, such as Helicobacter pylori. However, the dysregulation of miR-146a in H. pylori-infected gastric tumors has not been widely studied. We aimed to analyze the expression level of miR-146a in gastric cancer tissues and then to assess any potential association between miR-146a and H. pylori infection and other clinical characteristics. miR-146a expression level was quantitatively studied by reverse transcription quantitative polymerase chain reaction, in 144 fresh tissues including 44 normal and 100 gastric cancer samples. A dramatic overexpression of miR-146a was observed in primary gastric tumors. miR-146a showed lower expression in progressed tumors with greater stages and lymph node metastasis. miR-146a is highly expressed in primary gastric tumor independent of H. pylori infection. It is highly expressed in the lower stages and lymph node-negative tumors. It might suggest the importance of upregulation and downregulation of this miRNA in the initiating/promoting and progressive steps of gastric tumorigenesis, respectively.
Sections du résumé
BACKGROUND
BACKGROUND
Gastric cancer is one of the most significant reasons for cancer-related death. miR-146a is one of the dysregulated factors associated with gastric tumorigenesis. However, deregulation of this microRNA (miRNA) has become controversial. Moreover, the inflammation-mediating role of this miRNA implies that miR-146a might be dysregulated by gastric cancer-related pathogens, such as Helicobacter pylori. However, the dysregulation of miR-146a in H. pylori-infected gastric tumors has not been widely studied.
OBJECTIVES
OBJECTIVE
We aimed to analyze the expression level of miR-146a in gastric cancer tissues and then to assess any potential association between miR-146a and H. pylori infection and other clinical characteristics.
MATERIALS AND METHODS
METHODS
miR-146a expression level was quantitatively studied by reverse transcription quantitative polymerase chain reaction, in 144 fresh tissues including 44 normal and 100 gastric cancer samples.
RESULTS
RESULTS
A dramatic overexpression of miR-146a was observed in primary gastric tumors. miR-146a showed lower expression in progressed tumors with greater stages and lymph node metastasis.
CONCLUSION
CONCLUSIONS
miR-146a is highly expressed in primary gastric tumor independent of H. pylori infection. It is highly expressed in the lower stages and lymph node-negative tumors. It might suggest the importance of upregulation and downregulation of this miRNA in the initiating/promoting and progressive steps of gastric tumorigenesis, respectively.
Identifiants
pubmed: 30880764
pii: JCanResTher_2019_15_1_108_244479
doi: 10.4103/jcrt.JCRT_855_17
doi:
Substances chimiques
MIRN146 microRNA, human
0
MicroRNAs
0
Types de publication
Journal Article
Langues
eng
Pagination
108-114Déclaration de conflit d'intérêts
None