G-Quadruplexes in Human Ribosomal RNA.

Animals Base Sequence Circular Dichroism Electrophoretic Mobility Shift Assay G-Quadruplexes Humans Nucleic Acid Conformation RNA, Ribosomal / chemistry Ribosome Subunits, Large / chemistry Sequence Alignment
Chordates expansion segments helicases polysomes rRNA

Journal

Journal of molecular biology
ISSN: 1089-8638
Titre abrégé: J Mol Biol
Pays: Netherlands
ID NLM: 2985088R

Informations de publication

Date de publication:
03 05 2019
Historique:
received: 16 11 2018
revised: 07 03 2019
accepted: 08 03 2019
pubmed: 20 3 2019
medline: 24 3 2020
entrez: 20 3 2019
Statut: ppublish

Résumé

rRNA is the single most abundant polymer in most cells. Mammalian rRNAs are nearly twice as large as those of prokaryotes. Differences in rRNA size are due to expansion segments, which contain extended tentacles in metazoans. Here we show that the terminus of an rRNA tentacle of Homo sapiens contains 10 tandem G-tracts that form highly stable G-quadruplexes in vitro. We characterized rRNA of the H. sapiens large ribosomal subunit by computation, circular dichroism, UV melting, fluorescent probes, nuclease accessibility, electrophoretic mobility shifts, and blotting. We investigated Expansion Segment 7 (ES7), oligomers derived from ES7, intact 28S rRNA, 80S ribosomes, and polysomes. We used mass spectrometry to identify proteins that bind to rRNA G-quadruplexes in cell lysates. These proteins include helicases (DDX3, CNBP, DDX21, DDX17) and heterogeneous nuclear ribonucleoproteins. Finally, by multiple sequence alignments, we observe that G-quadruplex-forming sequences are a general feature of LSU rRNA of Chordata but not, as far as we can tell, of other species. Chordata ribosomes present polymorphic tentacles with the potential to switch between inter- and intramolecular G-quadruplexes. To our knowledge, G-quadruplexes have not been reported previously in ribosomes.

Identifiants

DOI: 10.1016/j.jmb.2019.03.010 PMID: 30885721 PMC: PMC8064279
pubmed: 30885721
pii: S0022-2836(19)30133-0
doi: 10.1016/j.jmb.2019.03.010
pmc: PMC8064279
mid: NIHMS1526157
pii:
doi:

Substances chimiques

RNA, Ribosomal 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

1940-1955

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM118803
Pays : United States

Informations de copyright

Copyright © 2019 Elsevier Ltd. All rights reserved.

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Auteurs

Santi Mestre-Fos (S)

Center for the Origin of Life, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA; School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA.

Petar I Penev (PI)

Center for the Origin of Life, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA; School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA.

Suttipong Suttapitugsakul (S)

School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA.

Michael Hu (M)

Center for the Origin of Life, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA; School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA.

Chieri Ito (C)

Center for the Origin of Life, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA; School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA.

Anton S Petrov (AS)

Center for the Origin of Life, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA; School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA.

Roger M Wartell (RM)

Center for the Origin of Life, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA; School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA; School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA.

Ronghu Wu (R)

School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA.

Loren Dean Williams (LD)

Center for the Origin of Life, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA; School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA; School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA. Electronic address: loren.williams@chemistry.gatech.edu.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › G-Quadruplexes in Human Ribosomal RNA.
questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence nucléotidique › G-Quadruplexes in Human Ribosomal RNA.
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Dichroïsme circulaire › G-Quadruplexes in Human Ribosomal RNA.
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Test de retard de migration électrophorétique › G-Quadruplexes in Human Ribosomal RNA.
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Conformation d'acide nucléique › G-quadruplexes › G-Quadruplexes in Human Ribosomal RNA.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › G-Quadruplexes in Human Ribosomal RNA.
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Conformation d'acide nucléique › G-Quadruplexes in Human Ribosomal RNA.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN ribosomique › G-Quadruplexes in Human Ribosomal RNA.
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Ribosomes › Sous-unités du ribosome › Grande sous-unité du ribosome › G-Quadruplexes in Human Ribosomal RNA.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Alignement de séquences › G-Quadruplexes in Human Ribosomal RNA.