A critical regulator of Bcl2 revealed by systematic transcript discovery of lncRNAs associated with T-cell differentiation.
Animals
Apoptosis
/ genetics
CRISPR-Cas Systems
/ genetics
Cell Differentiation
/ drug effects
Cell Line, Tumor
Gene Knockdown Techniques
Genetic Loci
Ionomycin
/ pharmacology
Lymphocyte Activation
/ drug effects
Mice
Proto-Oncogene Proteins c-bcl-2
/ genetics
RNA, Long Noncoding
/ genetics
T-Lymphocytes
/ physiology
Tetradecanoylphorbol Acetate
/ analogs & derivatives
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
18 03 2019
18 03 2019
Historique:
received:
08
10
2018
accepted:
01
03
2019
entrez:
20
3
2019
pubmed:
20
3
2019
medline:
21
10
2020
Statut:
epublish
Résumé
Normal T-cell differentiation requires a complex regulatory network which supports a series of maturation steps, including lineage commitment, T-cell receptor (TCR) gene rearrangement, and thymic positive and negative selection. However, the underlying molecular mechanisms are difficult to assess due to limited T-cell models. Here we explore the use of the pro-T-cell line P5424 to study early T-cell differentiation. Stimulation of P5424 cells by the calcium ionophore ionomycin together with PMA resulted in gene regulation of T-cell differentiation and activation markers, partially mimicking the CD4
Identifiants
pubmed: 30886319
doi: 10.1038/s41598-019-41247-5
pii: 10.1038/s41598-019-41247-5
pmc: PMC6423290
doi:
Substances chimiques
Proto-Oncogene Proteins c-bcl-2
0
RNA, Long Noncoding
0
Bcl2 protein, mouse
114100-40-2
Ionomycin
56092-81-0
phorbolol myristate acetate
56937-68-9
Tetradecanoylphorbol Acetate
NI40JAQ945
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4707Références
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