Validation of the CULTEX® Radial Flow System for the assessment of the acute inhalation toxicity of airborne particles.
Acute inhalation toxicity
Air-liquid interface
CULTEX® RFS
In vitro
Validation
Journal
Toxicology in vitro : an international journal published in association with BIBRA
ISSN: 1879-3177
Titre abrégé: Toxicol In Vitro
Pays: England
ID NLM: 8712158
Informations de publication
Date de publication:
Aug 2019
Aug 2019
Historique:
received:
10
12
2018
revised:
08
03
2019
accepted:
15
03
2019
pubmed:
21
3
2019
medline:
4
12
2019
entrez:
21
3
2019
Statut:
ppublish
Résumé
The CULTEX® Radial Flow System (RFS) is a modular in vitro system for the homogenous exposure of cells to airborne particles at the air-liquid interface (ALI). A former pre-validation study successfully demonstrated the general applicability of the CULTEX® RFS and its transferability, stability and reproducibility. Based on these results, the methodology was optimized, validated and prediction models for acute inhalation hazards were established. Cell viability of A549 cells after ALI exposure to 20 pre-selected test substances was assessed in three independent laboratories. Cytotoxicity of test substances was compared to the respective incubator controls and used as an indicator of toxicity. Substances were considered to exert an acute inhalation hazard when viability decreased below 50% (prediction model (PM) 50%) or 75% (PM 75%) at any of three exposure doses (25, 50 or 100 μg/cm
Identifiants
pubmed: 30890356
pii: S0887-2333(18)30811-7
doi: 10.1016/j.tiv.2019.03.020
pii:
doi:
Substances chimiques
Air Pollutants
0
Particulate Matter
0
Types de publication
Journal Article
Validation Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
245-255Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.