Metabarcoding for stomach-content analyses of Pygmy devil ray (
DNA extraction
DNA metabarcoding
Illumina sequencing
marine predator
stomach-content
Journal
Ecology and evolution
ISSN: 2045-7758
Titre abrégé: Ecol Evol
Pays: England
ID NLM: 101566408
Informations de publication
Date de publication:
Mar 2019
Mar 2019
Historique:
received:
11
09
2018
revised:
22
11
2018
accepted:
28
12
2018
entrez:
21
3
2019
pubmed:
21
3
2019
medline:
21
3
2019
Statut:
epublish
Résumé
The application of high-throughput sequencing to retrieve multi-taxon DNA from different substrates such as water, soil, and stomach contents has enabled species identification without prior knowledge of taxon compositions. Here we used three minibarcodes designed to target mitochondrial COI in plankton, 16S in fish, and 16S in crustaceans, to compare ethanol- and tissue-derived DNA extraction methodologies for metabarcoding. The stomach contents of pygmy devilrays (
Identifiants
pubmed: 30891208
doi: 10.1002/ece3.4934
pii: ECE34934
pmc: PMC6405894
doi:
Banques de données
Dryad
['10.5061/dryad.288vb66']
Types de publication
Journal Article
Langues
eng
Pagination
2678-2687Déclaration de conflit d'intérêts
None declared.
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