In situ microscopy as online tool for detecting microbial contaminations in cell culture.
Candida
Hybridoma cells
Image analysis
Infection
Mammalian cell culture
Pichia
Journal
Journal of biotechnology
ISSN: 1873-4863
Titre abrégé: J Biotechnol
Pays: Netherlands
ID NLM: 8411927
Informations de publication
Date de publication:
20 Apr 2019
20 Apr 2019
Historique:
received:
20
06
2018
revised:
14
03
2019
accepted:
15
03
2019
pubmed:
23
3
2019
medline:
28
7
2019
entrez:
23
3
2019
Statut:
ppublish
Résumé
Microbial contamination in mammalian cell cultures causing rejected batches is costly and highly unwanted. Most methods for detecting a contamination are time-consuming and require extensive off-line sampling. To circumvent these efforts and provide a more convenient alternative, we used an online in situ microscope to estimate the cell diameter of the cellular species in the culture to distinguish mammalian cells from microbial cells depending on their size. A warning system was set up to alert the operator if microbial cells were present in the culture. Hybridoma cells were cultured and infected with either Candida utilis or Pichia stipitis as contaminant. The warning system could successfully detect the introduced contamination and alert the operator. The results suggest that in situ microscopy could be used as an efficient online tool for early detection of contaminations in cell cultures.
Identifiants
pubmed: 30898686
pii: S0168-1656(19)30084-7
doi: 10.1016/j.jbiotec.2019.03.011
pii:
doi:
Substances chimiques
Culture Media
0
Types de publication
Journal Article
Langues
eng
Pagination
53-60Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.