Autologous grafting of cryopreserved prepubertal rhesus testis produces sperm and offspring.
Journal
Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511
Informations de publication
Date de publication:
22 03 2019
22 03 2019
Historique:
received:
03
09
2018
accepted:
13
02
2019
entrez:
23
3
2019
pubmed:
23
3
2019
medline:
24
8
2019
Statut:
ppublish
Résumé
Testicular tissue cryopreservation is an experimental method to preserve the fertility of prepubertal patients before they initiate gonadotoxic therapies for cancer or other conditions. Here we provide the proof of principle that cryopreserved prepubertal testicular tissues can be autologously grafted under the back skin or scrotal skin of castrated pubertal rhesus macaques and matured to produce functional sperm. During the 8- to 12-month observation period, grafts grew and produced testosterone. Complete spermatogenesis was confirmed in all grafts at the time of recovery. Graft-derived sperm were competent to fertilize rhesus oocytes, leading to preimplantation embryo development, pregnancy, and the birth of a healthy female baby. Pending the demonstration that similar results are obtained in noncastrated recipients, testicular tissue grafting may be applied in the clinic.
Identifiants
pubmed: 30898927
pii: 363/6433/1314
doi: 10.1126/science.aav2914
pmc: PMC6598202
mid: NIHMS1023184
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
1314-1319Subventions
Organisme : NICHD NIH HHS
ID : P01 HD075795
Pays : United States
Organisme : NIH HHS
ID : P51 OD011092
Pays : United States
Organisme : NICHD NIH HHS
ID : R01 HD055475
Pays : United States
Organisme : NICHD NIH HHS
ID : R01 HD076412
Pays : United States
Commentaires et corrections
Type : CommentIn
Type : ErratumIn
Type : CommentIn
Type : CommentIn
Informations de copyright
Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
Références
Biol Reprod. 2003 Jun;68(6):2331-5
pubmed: 12606381
Obstet Gynecol. 2010 Nov;116(5):1171-83
pubmed: 20966703
Am J Primatol. 1991;24(1):29-38
pubmed: 31952393
Retrovirology. 2006 Oct 03;3:67
pubmed: 17018135
Hum Reprod. 2002 Dec;17(12):3039-45
pubmed: 12456600
Nat Protoc. 2010 Jun;5(6):1138-47
pubmed: 20539289
Hum Reprod. 2013 Apr;28(4):897-907
pubmed: 23427228
Reproduction. 2014 Nov;148(5):R71-84
pubmed: 25150043
Pediatr Blood Cancer. 2009 Aug;53(2):261-6
pubmed: 19326418
Lancet Oncol. 2005 Apr;6(4):209-18
pubmed: 15811616
Hum Reprod. 1998 Dec;13(12):3449-55
pubmed: 9886532
J Clin Oncol. 2010 Nov 10;28(32):4831-41
pubmed: 20458029
Microvasc Res. 1998 Jul;56(1):30-42
pubmed: 9683561
J Clin Oncol. 2010 Jan 10;28(2):332-9
pubmed: 19949008
Biol Reprod. 2006 Apr;74(4):706-13
pubmed: 16371588
J Med Primatol. 1986;15(5):351-9
pubmed: 3100808
Cell Res. 2016 Jan;26(1):139-42
pubmed: 26369429
Hum Reprod. 2005 Jun;20(6):1676-87
pubmed: 15860503
Hum Reprod. 2007 May;22(5):1384-95
pubmed: 17259225
Hum Reprod Update. 2016 Nov;22(6):744-761
pubmed: 27566839
Fertil Steril. 2000 May;73(5):955-60
pubmed: 10785220
Cancer Res. 2012 Oct 15;72(20):5174-8
pubmed: 22902414
Nature. 2002 Aug 15;418(6899):778-81
pubmed: 12181567
Fertil Steril. 2016 Feb;105(2):256-66
pubmed: 26746133
Reprod Suppl. 2001;58:223-32
pubmed: 11980192
Dev Neurosci. 2011;33(3-4):210-21
pubmed: 21659720
J Anat. 1984 Oct;139 ( Pt 3):535-52
pubmed: 6490534
Biol Reprod. 1978 Jun;18(5):749-53
pubmed: 96882
Hum Reprod. 2007 Apr;22(4):1060-7
pubmed: 17166865
Biol Reprod. 2004 May;70(5):1500-3
pubmed: 14736818
Hum Reprod. 2001 Jan;16(1):130-137
pubmed: 11139551
Mol Reprod Dev. 1990 Nov;27(3):261-80
pubmed: 2078341
Endocrinology. 2008 Apr;149(4):1736-47
pubmed: 18174281
J Med Primatol. 1972;1(1):2-28
pubmed: 5073900
Viruses. 2014 May 13;6(5):2062-83
pubmed: 24828841
Fertil Steril. 2014 Jan;101(1):3-13
pubmed: 24314923
Endocrinology. 1992 Mar;130(3):1238-44
pubmed: 1537290
Hum Reprod. 2006 Jun;21(6):1368-79
pubmed: 16459346
PLoS One. 2013 Jul 29;8(7):e70989
pubmed: 23923039
Lancet. 2002 Aug 3;360(9330):361-7
pubmed: 12241775
J Reprod Infertil. 2013 Apr;14(2):79-84
pubmed: 23926569
Comp Med. 2008 Dec;58(6):597-603
pubmed: 19149417