A post-translational modification of human Norovirus capsid protein attenuates glycan binding.
Asparagine
/ chemistry
Binding Sites
Blood Group Antigens
/ chemistry
Capsid Proteins
/ chemistry
Cloning, Molecular
Crystallography, X-Ray
Escherichia coli
/ genetics
Gene Expression
Genetic Vectors
/ chemistry
Host-Pathogen Interactions
Humans
Isoaspartic Acid
/ chemistry
Kinetics
Models, Molecular
Norovirus
/ genetics
Polysaccharides
/ chemistry
Protein Binding
Protein Conformation, alpha-Helical
Protein Conformation, beta-Strand
Protein Interaction Domains and Motifs
Protein Multimerization
Protein Processing, Post-Translational
Recombinant Proteins
/ chemistry
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
21 03 2019
21 03 2019
Historique:
received:
12
09
2018
accepted:
27
02
2019
entrez:
23
3
2019
pubmed:
23
3
2019
medline:
5
4
2019
Statut:
epublish
Résumé
Attachment of human noroviruses to histo blood group antigens (HBGAs) is essential for infection, but how this binding event promotes the infection of host cells is unknown. Here, we employ protein NMR experiments supported by mass spectrometry and crystallography to study HBGA binding to the P-domain of a prevalent virus strain (GII.4). We report a highly selective transformation of asparagine 373, located in an antigenic loop adjoining the HBGA binding site, into an iso-aspartate residue. This spontaneous post-translational modification (PTM) proceeds with an estimated half-life of a few days at physiological temperatures, independent of the presence of HBGAs but dramatically affecting HBGA recognition. Sequence conservation and the surface-exposed position of this PTM suggest an important role in infection and immune recognition for many norovirus strains.
Identifiants
pubmed: 30899001
doi: 10.1038/s41467-019-09251-5
pii: 10.1038/s41467-019-09251-5
pmc: PMC6428809
doi:
Substances chimiques
Blood Group Antigens
0
Capsid Proteins
0
Isoaspartic Acid
0
Polysaccharides
0
Recombinant Proteins
0
Asparagine
7006-34-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1320Références
J Virol. 2014 Aug;88(16):8826-42
pubmed: 24872579
Angew Chem Int Ed Engl. 2010 Nov 2;49(45):8421-5
pubmed: 20878961
Cell. 2002 Oct 4;111(1):51-62
pubmed: 12372300
Nucleic Acids Res. 2017 Jan 4;45(D1):D1100-D1106
pubmed: 27924013
MAbs. 2015;7(3):525-39
pubmed: 25875351
Biochemistry. 2018 Jan 16;57(2):177-185
pubmed: 29064683
Curr Opin Virol. 2018 Aug;31:33-42
pubmed: 29754860
J Virol. 2015 Feb;89(4):2024-40
pubmed: 25428879
Glycobiology. 2017 Jan;27(1):80-86
pubmed: 27496762
mSphere. 2018 Feb 7;3(1):
pubmed: 29435493
Anal Chem. 2016 Feb 16;88(4):2041-50
pubmed: 26824491
Nat Protoc. 2015 Dec;10(12):1939-47
pubmed: 26513671
J Virol. 2012 Jan;86(1):284-92
pubmed: 22031945
J Virol. 2007 Sep;81(18):9932-41
pubmed: 17609280
J Mol Biol. 1998 Apr 24;278(1):231-8
pubmed: 9571046
Nucleic Acids Res. 2016 Jan 4;44(D1):D447-56
pubmed: 26527722
Acta Crystallogr D Biol Crystallogr. 2012 Apr;68(Pt 4):431-40
pubmed: 22505263
Proteins. 2005 Jun 1;59(4):687-96
pubmed: 15815974
J Virol. 2007 Jun;81(11):5949-57
pubmed: 17392366
Hum Vaccin Immunother. 2014;10(6):1449-56
pubmed: 24718366
Biochemistry. 1989 Oct 17;28(21):8646-53
pubmed: 2605213
Nucleic Acids Res. 2008 Jan;36(Database issue):D402-8
pubmed: 17984079
J Virol. 2009 Nov;83(22):11890-901
pubmed: 19759138
Curr Opin Struct Biol. 2017 Jun;44:190-200
pubmed: 28544984
Acta Crystallogr D Biol Crystallogr. 1997 May 1;53(Pt 3):240-55
pubmed: 15299926
Proc Natl Acad Sci U S A. 2001 Oct 23;98(22):12409-13
pubmed: 11606750
Nucleic Acids Res. 2007 Jul;35(Web Server issue):W375-83
pubmed: 17452350
Prog Nucl Magn Reson Spectrosc. 2013 Aug;73:1-16
pubmed: 23962882
Chemistry. 2011 Jun 27;17(27):7438-41
pubmed: 21469230
Glycobiology. 2017 Nov 1;27(11):1027-1037
pubmed: 28973640
J Am Chem Soc. 2002 Aug 28;124(34):10025-35
pubmed: 12188667
Antimicrob Agents Chemother. 2007 Jan;51(1):324-31
pubmed: 17060523
Prog Nucl Magn Reson Spectrosc. 2014 Apr;78:47-75
pubmed: 24534088
Virology. 2015 Sep;483:203-8
pubmed: 25980740
Nat Rev Microbiol. 2010 Mar;8(3):231-41
pubmed: 20125087
J Cell Sci. 2011 Feb 15;124(Pt 4):515-22
pubmed: 21282473
J Mol Biol. 2001 Nov 9;313(5):1103-16
pubmed: 11700066
Mass Spectrom Rev. 2017 Nov;36(6):677-692
pubmed: 26763661
Chemistry. 2011 Jun 27;17(27):7442-53
pubmed: 21567493
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):133-44
pubmed: 20124693
Anal Chem. 2017 Nov 21;89(22):11962-11970
pubmed: 29058416
J Virol. 2004 Jun;78(12):6233-42
pubmed: 15163716
Glycoconj J. 2017 Oct;34(5):679-689
pubmed: 28823097
Proc Natl Acad Sci U S A. 2001 Apr 10;98(8):4367-72
pubmed: 11296285
Proc Natl Acad Sci U S A. 1987 May;84(9):2595-9
pubmed: 3472227
mBio. 2013 Jul 16;4(4):
pubmed: 23860770
Science. 2014 Nov 7;346(6210):755-9
pubmed: 25378626
Anal Chem. 2009 Oct 1;81(19):7870-5
pubmed: 19788312
Virology. 2017 Aug;508:81-89
pubmed: 28505592
J Phys Chem B. 2017 Feb 2;121(4):719-730
pubmed: 28051868
Nat Rev Microbiol. 2016 Jul;14(7):421-33
pubmed: 27211790
J Biomol NMR. 2013 Jul;56(3):227-41
pubmed: 23728592
Nat Commun. 2016 Sep 28;7:12798
pubmed: 27677693
Curr Opin Struct Biol. 2017 Jun;44:211-218
pubmed: 28591681
Protein Expr Purif. 2011 Feb;75(2):192-203
pubmed: 20851186
Chem Commun (Camb). 2018 May 8;54(38):4761-4769
pubmed: 29662983
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32
pubmed: 15572765
PLoS One. 2013 Jul 19;8(7):e69379
pubmed: 23894462
Nat Protoc. 2016 Dec;11(12):2301-2319
pubmed: 27809316
Proc Natl Acad Sci U S A. 2001 Jan 30;98(3):944-9
pubmed: 11158575
J Biomol NMR. 2008 May;41(1):5-7
pubmed: 18398685
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21
pubmed: 20124702
J Biol Chem. 1987 Jan 15;262(2):785-94
pubmed: 3805008
J Virol. 2011 Jul;85(13):6687-701
pubmed: 21525337
Front Pharmacol. 2014 Apr 29;5:87
pubmed: 24808864
Annu Rev Biophys Biomol Struct. 1998;27:357-406
pubmed: 9646872
Science. 2016 Sep 23;353(6306):1387-1393
pubmed: 27562956
Nat Commun. 2012 Jun 26;3:927
pubmed: 22735455
J Magn Reson. 2006 Jan;178(1):72-6
pubmed: 16188473
Angew Chem Int Ed Engl. 2015 Oct 5;54(41):12014-9
pubmed: 26329854
Proc Natl Acad Sci U S A. 1970 Jul;66(3):753-7
pubmed: 5269237
PLoS Med. 2008 Feb;5(2):e31
pubmed: 18271619