Α 2-stage, nested-like nucleic acid amplification method (IsoPCR) for the highly sensitive detection of HPV16 and HPV18 DNA.
DNA, Viral
/ genetics
DNA-Binding Proteins
/ genetics
Female
Human papillomavirus 16
/ genetics
Human papillomavirus 18
/ genetics
Humans
Molecular Diagnostic Techniques
/ methods
Nucleic Acid Amplification Techniques
/ methods
Oncogene Proteins, Viral
/ genetics
Papillomavirus Infections
/ diagnosis
Polymerase Chain Reaction
/ methods
Repressor Proteins
/ genetics
Sensitivity and Specificity
Uterine Cervical Neoplasms
/ virology
Cervical dysplasia
HPV genotyping
HPV16
HPV18
IsoPCR
LAMP
Journal
Molecular and cellular probes
ISSN: 1096-1194
Titre abrégé: Mol Cell Probes
Pays: England
ID NLM: 8709751
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
14
11
2018
revised:
20
01
2019
accepted:
14
03
2019
pubmed:
25
3
2019
medline:
30
4
2020
entrez:
24
3
2019
Statut:
ppublish
Résumé
Molecular detection of HPV DNA is considered as the gold standard for the diagnosis of cervical disease. Although the molecular assays for the identification of HPV16 and HPV18 have helped identify cervical cancer incidents, they are restricted to specialized laboratories. Thus, we developed a novel 2-stage, nested-like nucleic acid amplification method, named IsoPCR, to amplify the E6 gene of HPV16 and HPV18 with high analytical sensitivity and specificity. The performance of IsoPCR was compared to that of conventional PCR and LAMP. The analytical sensitivity of IsoPCR (1 copy/test) was 10-fold higher than conventional PCR and 25-fold higher than conventional LAMP. IsoPCR displayed significant amplification specificity (100%) and efficiency, as well. In conclusion, IsoPCR is a highly sensitive and specific diagnostic tool and it is suitable for the detection of low copy number of viral DNA in clinical specimens, providing critical information to healthcare providers.
Identifiants
pubmed: 30902662
pii: S0890-8508(18)30307-4
doi: 10.1016/j.mcp.2019.03.003
pii:
doi:
Substances chimiques
DNA, Viral
0
DNA-Binding Proteins
0
E6 protein, Human papillomavirus type 16
0
E6 protein, Human papillomavirus type 18
0
Oncogene Proteins, Viral
0
Repressor Proteins
0
Types de publication
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1-7Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.