Assessment of induced genomic instability in rat primary astrocytes exposed to intermediate frequency magnetic fields.
Comet assay
Induced genomic instability
Intermediate frequency
Magnetic fields
Micronucleus
Journal
Environmental research
ISSN: 1096-0953
Titre abrégé: Environ Res
Pays: Netherlands
ID NLM: 0147621
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
16
11
2018
revised:
13
03
2019
accepted:
14
03
2019
pubmed:
25
3
2019
medline:
3
1
2020
entrez:
24
3
2019
Statut:
ppublish
Résumé
We investigated whether exposure to intermediate frequency magnetic fields (IF MFs) could induce or enhance genomic instability in primary astrocytes. Rat primary astrocytes were exposed to vertical or horizontal 7.5 kHz, 300 μT MF for 24 h. To study possible combined effects with known genotoxic agents, the cells were exposed for 3 h to menadione or methyl methanesulfonate after the MF treatment. Induced genomic instability was evaluated 36 days after exposures using the Comet assay and flow cytometric scoring of micronuclei. Exposure to 7.5 kHz, 300 μT MF did not induce genomic instability alone or in combination with chemicals in measurements performed several cell generations after exposure.
Identifiants
pubmed: 30903815
pii: S0013-9351(19)30161-6
doi: 10.1016/j.envres.2019.03.033
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
112-116Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.