A novel beta-1,4 glucanase produced by symbiotic Bacillus sp. CF96 isolated from termite (Anacanthotermes).
Bacillus
Beta-1,4-glucanase
Salt sensitive
Termite
Thermophile
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
15 Jun 2019
15 Jun 2019
Historique:
received:
21
01
2019
revised:
19
03
2019
accepted:
19
03
2019
pubmed:
25
3
2019
medline:
10
9
2019
entrez:
25
3
2019
Statut:
ppublish
Résumé
A novel beta-1,4-glucanase was purified and characterized from symbiotic Bacillus sp. CF96 of termite. The SDS-PAGE and zymogram analyses revealed a molecular mass of 35.6 kDa. Optimal activity was at 50 °C and pH 5.5, while the enzyme was active over a wide range of temperature 20-80 °C and pH 4-10 and interestingly more than 60% of the maximum activity remained up to pH 9. The enzyme activity increased in the presence of hexane, chloroform and methanol (20% v/v). while, the enzyme activity was inhibited by metal ions such as Mn
Identifiants
pubmed: 30904535
pii: S0141-8130(19)30512-4
doi: 10.1016/j.ijbiomac.2019.03.124
pii:
doi:
Substances chimiques
Solvents
0
Glycoside Hydrolases
EC 3.2.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
752-759Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.