Ten years of external quality assessment (EQA) of Neisseria gonorrhoeae antimicrobial susceptibility testing in Europe elucidate high reliability of data.


Journal

BMC infectious diseases
ISSN: 1471-2334
Titre abrégé: BMC Infect Dis
Pays: England
ID NLM: 100968551

Informations de publication

Date de publication:
25 Mar 2019
Historique:
received: 08 01 2019
accepted: 12 03 2019
entrez: 27 3 2019
pubmed: 27 3 2019
medline: 1 5 2019
Statut: epublish

Résumé

Confidence in any diagnostic and antimicrobial susceptibility testing data is provided by appropriate and regular quality assurance (QA) procedures. In Europe, the European Gonococcal Antimicrobial Susceptibility Programme (Euro-GASP) has been monitoring the antimicrobial susceptibility in Neisseria gonorrhoeae since 2004. Euro-GASP includes an external quality assessment (EQA) scheme as an essential component for a quality-assured laboratory-based surveillance programme. Participation in the EQA scheme enables any problems with the performed antimicrobial susceptibility testing to be identified and addressed, feeds into the curricula of laboratory training organised by the Euro-GASP network, and assesses the capacity of individual laboratories to detect emerging new, rare and increasing antimicrobial resistance phenotypes. Participant performance in the Euro-GASP EQA scheme over a 10 year period (2007 to 2016, no EQA in 2013) was evaluated. Antimicrobial susceptibility category and MIC results from the first 5 years (2007-2011) of the Euro-GASP EQA were compared with the latter 5 years (2012-2016). These time periods were selected to assess the impact of the 2012 European Union case definitions for the reporting of antimicrobial susceptibility. Antimicrobial susceptibility category agreement in each year was ≥91%. Discrepancies in susceptibility categories were generally because the MICs for EQA panel isolates were on or very close to the susceptibility or resistance breakpoints. A high proportion of isolates tested over the 10 years were within one (≥90%) or two (≥97%) MIC log The high level of comparability of results in this EQA scheme indicates that high quality data are produced by the Euro-GASP participants and gives confidence in susceptibility and resistance data generated by laboratories performing decentralised testing.

Sections du résumé

BACKGROUND BACKGROUND
Confidence in any diagnostic and antimicrobial susceptibility testing data is provided by appropriate and regular quality assurance (QA) procedures. In Europe, the European Gonococcal Antimicrobial Susceptibility Programme (Euro-GASP) has been monitoring the antimicrobial susceptibility in Neisseria gonorrhoeae since 2004. Euro-GASP includes an external quality assessment (EQA) scheme as an essential component for a quality-assured laboratory-based surveillance programme. Participation in the EQA scheme enables any problems with the performed antimicrobial susceptibility testing to be identified and addressed, feeds into the curricula of laboratory training organised by the Euro-GASP network, and assesses the capacity of individual laboratories to detect emerging new, rare and increasing antimicrobial resistance phenotypes. Participant performance in the Euro-GASP EQA scheme over a 10 year period (2007 to 2016, no EQA in 2013) was evaluated.
METHODS METHODS
Antimicrobial susceptibility category and MIC results from the first 5 years (2007-2011) of the Euro-GASP EQA were compared with the latter 5 years (2012-2016). These time periods were selected to assess the impact of the 2012 European Union case definitions for the reporting of antimicrobial susceptibility.
RESULTS RESULTS
Antimicrobial susceptibility category agreement in each year was ≥91%. Discrepancies in susceptibility categories were generally because the MICs for EQA panel isolates were on or very close to the susceptibility or resistance breakpoints. A high proportion of isolates tested over the 10 years were within one (≥90%) or two (≥97%) MIC log
CONCLUSIONS CONCLUSIONS
The high level of comparability of results in this EQA scheme indicates that high quality data are produced by the Euro-GASP participants and gives confidence in susceptibility and resistance data generated by laboratories performing decentralised testing.

Identifiants

pubmed: 30909883
doi: 10.1186/s12879-019-3900-z
pii: 10.1186/s12879-019-3900-z
pmc: PMC6434634
doi:

Substances chimiques

Anti-Bacterial Agents 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

281

Subventions

Organisme : European Centre for Disease Prevention and Control
ID : ECDC/2013/015

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Auteurs

Michelle J Cole (MJ)

Antimicrobial Resistance and Healthcare Associated Infections (AMRHAI) Reference Unit, National Infection Service, Public Health England, London, UK. michelle.cole@phe.gov.uk.

Nerteley Quaye (N)

National Mycobacterium Reference Service, National Infection Service, Public Health England, London, UK.

Susanne Jacobsson (S)

WHO Collaborating Centre for Gonorrhoea and other STIs, Örebro University, Örebro, Sweden.

Michaela Day (M)

Antimicrobial Resistance and Healthcare Associated Infections (AMRHAI) Reference Unit, National Infection Service, Public Health England, London, UK.

Elizabeth Fagan (E)

United Kingdom National External Quality Assessment Service (UK NEQAS), National Infection Service, Public Health England, London, UK.

Catherine Ison (C)

Sexually Transmitted Bacteria Reference Unit (retired), Public Health England, London, UK.

Rachel Pitt (R)

Antimicrobial Resistance and Healthcare Associated Infections (AMRHAI) Reference Unit, National Infection Service, Public Health England, London, UK.

Shila Seaton (S)

United Kingdom National External Quality Assessment Service (UK NEQAS), National Infection Service, Public Health England, London, UK.

Neil Woodford (N)

Antimicrobial Resistance and Healthcare Associated Infections (AMRHAI) Reference Unit, National Infection Service, Public Health England, London, UK.

Angelika Stary (A)

Outpatients` Centre for the Diagnosis of Infectious Venero-Dermatological Diseases, Vienna, Austria.

Sonja Pleininger (S)

National Reference Centre for Gonococci, Austrian Agency for Health and Food Safety, Vienna, Austria.

Tania Crucitti (T)

Institute of Tropical Medicine, Antwerp, Belgium.

Blaženka Hunjak (B)

Croatian Institute of Public Health, Zagreb, Croatia.

Panayiota Maikanti (P)

Microbiology Department, Nicosia General Hospital, Nicosia, Cyprus.

Steen Hoffmann (S)

Department for Bacteria, Parasites and Fungi Infectious Diseases Preparedness, Statens Serum Institut, Copenhagen, Denmark.

Jelena Viktorova (J)

Central Laboratory of Communicable Diseases, Tallinn, Estonia.

Susanne Buder (S)

Laboratory for Gonococci, Vivantes Klinikum, South, Berlin, Germany.

Peter Kohl (P)

Laboratory for Gonococci, Vivantes Klinikum, South, Berlin, Germany.

Eva Tzelepi (E)

National Reference Centre for N. gonorrhoeae, Laboratory of Bacteriology of the Hellenic Pasteur Institute, Athens, Greece.

Eirini Siatravani (E)

National Reference Centre for N. gonorrhoeae, Laboratory of Bacteriology of the Hellenic Pasteur Institute, Athens, Greece.

Eszter Balla (E)

Bacterial STI Reference Laboratory, National Public Health Institute, Budapest, Hungary.

Guðrún Svanborg Hauksdóttir (GS)

Landspitali University Hospital, Reykjavik, Iceland.

Lisa Rose (L)

National Gonococcal Reference Laboratory, St James's Hospital, Dublin, Ireland.

Paola Stefanelli (P)

Department of Infectious Diseases, Istituto Superiore di Sanità, Rome, Italy.

Anna Carannante (A)

Department of Infectious Diseases, Istituto Superiore di Sanità, Rome, Italy.

Gatis Pakarna (G)

National Microbiology Reference Laboratory, Latvian Centre of Infectious Diseases, Riga East University Hospital, Riga, Latvia.

Francesca Mifsud (F)

Bacteriology Laboratory, Mater Dei Hospital, Msida, Malta.

Rosann Zammit Cassar (RZ)

Bacteriology Laboratory, Mater Dei Hospital, Msida, Malta.

Ineke Linde (I)

Streeklaboratorium/Bacteriologie, GGD Amsterdam, Amsterdam, The Netherlands.

Thea Bergheim (T)

Department of Medical Microbiology, Oslo University Hospital, Oslo, Norway.

Martin Steinbakk (M)

Norwegian Institute of Public Health, Oslo, Norway.

Beata Mlynarczyk-Bonikowska (B)

Department of Diagnostics of Sexually Transmitted Diseases, Medical University of Warsaw, Warsaw, Poland.

Maria-José Borrego (MJ)

Instituto Nacional de Saúde Doutor Ricardo Jorge, Lisbon, Portugal.

Jill Shepherd (J)

Scottish Bacterial Sexually Transmitted Infections Reference Laboratory, Royal Infirmary of Edinburgh, Edinburgh, UK.

Peter Pavlik (P)

Medirex a.s., Bratislava, Slovakia.

Samo Jeverica (S)

Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.

Julio Vazquez (J)

Reference Laboratory for Neisseria National Centre for Microbiology - Instituto de Salud Carlos III, Majadahonda, Spain.

Raquel Abad (R)

Reference Laboratory for Neisseria National Centre for Microbiology - Instituto de Salud Carlos III, Majadahonda, Spain.

Sabrina Weiss (S)

Antimicrobial Resistance and Healthcare Associated Infections (AMRHAI) Reference Unit, National Infection Service, Public Health England, London, UK.
Institute of Virology, Charite - Universitätsmedizin Berlin, Berlin, Germany.
European Centre for Disease Prevention and Control, Stockholm, Sweden.

Gianfranco Spiteri (G)

European Centre for Disease Prevention and Control, Stockholm, Sweden.

Magnus Unemo (M)

WHO Collaborating Centre for Gonorrhoea and other STIs, Örebro University, Örebro, Sweden.

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