Omics of the early molecular dialogue between Frankia alni and Alnus glutinosa and the cellulase synton.
Journal
Environmental microbiology
ISSN: 1462-2920
Titre abrégé: Environ Microbiol
Pays: England
ID NLM: 100883692
Informations de publication
Date de publication:
Sep 2019
Sep 2019
Historique:
revised:
19
03
2019
received:
13
02
2019
accepted:
22
03
2019
pubmed:
28
3
2019
medline:
28
3
2019
entrez:
28
3
2019
Statut:
ppublish
Résumé
The early Frankia-Alnus symbiotic molecular exchanges were analyzed in detail by protein and RNA omics. For this, Frankia cells were placed in the presence of Alnus roots but separated by a dialysis membrane for 64 h. The bacterial cells were then harvested and analyzed by high-throughput proteomics and transcriptomics (RNA-seq). The most upregulated gene clusters were found to be the potassium transporter operon kdp and an ABC transporter operon of uncharacterized function. The most upregulated proteins were found to be acyl dehydrogenases and the potassium transporter Kdp. These suggest a preadaptation to the impending stresses linked to the penetration into isotonic host tissues and a possible rearrangement of the membrane. Another cluster among the 60 most upregulated ones that comprised two cellulases and a cellulose synthase was conserved among the Frankia and other actinobacteria such as Streptomyces. Cellulase activity was detected on CMC all along the length of the root but not away from it. Frankia alni ACN14a was found to be unable to respire or grow on glucose as sole carbon source. The cellulose synthase was found active at the tip of hyphae in response to Alnus root exudates, resulting in a calcofluor stained tip.
Identifiants
pubmed: 30917411
doi: 10.1111/1462-2920.14606
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3328-3345Subventions
Organisme : Agence Nationale de la Recherche
ID : ANR-10-BLAN-1708
Organisme : Agence Nationale de la Recherche
ID : ANR-13-BSV7-0013-03
Organisme : Université de Lyon
Informations de copyright
© 2019 Society for Applied Microbiology and John Wiley & Sons Ltd.
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