OMIP-056: Evaluation of Human Conventional T Cells, Donor-Unrestricted T Cells, and NK Cells Including Memory Phenotype by Intracellular Cytokine Staining.
AIDS Vaccines
/ immunology
Adult
Antigens
/ metabolism
Cytokines
/ metabolism
Flow Cytometry
/ methods
Fluorescent Dyes
/ chemistry
Humans
Immunologic Memory
Killer Cells, Natural
/ cytology
Leukocytes, Mononuclear
/ metabolism
Malaria Vaccines
/ immunology
Natural Killer T-Cells
/ cytology
T-Lymphocytes
/ cytology
Tuberculosis
/ immunology
MAIT cells
NK cells
T cells
cytometry
human PBMC
intracellular cytokines
memory
γδ T cells
Journal
Cytometry. Part A : the journal of the International Society for Analytical Cytology
ISSN: 1552-4930
Titre abrégé: Cytometry A
Pays: United States
ID NLM: 101235694
Informations de publication
Date de publication:
07 2019
07 2019
Historique:
received:
17
08
2018
revised:
02
12
2018
accepted:
19
02
2019
pubmed:
29
3
2019
medline:
28
8
2020
entrez:
29
3
2019
Statut:
ppublish
Résumé
A 26-color staining panel was developed to profile human antigen-specific T cells in an intracellular cytokine staining (ICS) assay using peptide pools to various antigens of interest. In addition to multiple functional markers, the panel includes differentiation/activation markers and markers to assess γδ, mucosal-associated invariant T, and NK T cells as well as conventional NK cells. Panel optimization was performed using previously cryopreserved PBMC from healthy adults, and then, expression of key functional markers in the panel was cross-validated against a validated ICS assay used in the HIV Vaccine Trials Network (HVTN). The panel is currently being used to evaluate the responses to tuberculosis and malaria vaccine candidates in volunteers from different geographic areas. © 2019 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry.
Identifiants
pubmed: 30919583
doi: 10.1002/cyto.a.23753
pmc: PMC6663658
mid: NIHMS1018447
doi:
Substances chimiques
AIDS Vaccines
0
Antigens
0
Cytokines
0
Fluorescent Dyes
0
Malaria Vaccines
0
Types de publication
Evaluation Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
722-725Subventions
Organisme : NIAID NIH HHS
ID : UM1 AI068618
Pays : United States
Organisme : NIAID NIH HHS
ID : P30 AI027757
Pays : United States
Commentaires et corrections
Type : ErratumIn
Informations de copyright
© 2019 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry.
Références
Cytometry A. 2012 Dec;81(12):1019-21
pubmed: 23081852
Cytometry A. 2015 Apr;87(4):289-92
pubmed: 25407958