Two-Photon Probes for Golgi Apparatus: Detection of Golgi Apparatus in Live Tissue by Two-Photon Microscopy.

Animals Apoptosis / physiology Benzoxazoles / chemical synthesis Drug Stability Fluorescent Dyes / chemical synthesis Golgi Apparatus / metabolism HeLa Cells Humans Microscopy, Fluorescence / methods Oligopeptides / chemical synthesis Photons Rats, Sprague-Dawley

Journal

Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536

Informations de publication

Date de publication:
21 05 2019
Historique:
pubmed: 29 3 2019
medline: 26 9 2020
entrez: 29 3 2019
Statut: ppublish

Résumé

We have developed blue- and yellow-emitting two-photon probes (BGolgi-blue and PGolgi-yellow) from 6-(benzo[ d]oxazol-2-yl)-2-naphthalylamine and 2,5-bis(benzo[ d]oxazol-2-yl)pyrazine derivatives as the fluorophores and trans-Golgi-network peptide (SDYQRL) as the Golgi-apparatus-targeting moiety. HeLa cells labeled with BGolgi-blue and PGolgi-yellow emitted two-photon-excited fluorescence at 462 and 560 nm, respectively, with effective two-photon-action cross-section values of 1860 and 1600 × 10

Identifiants

DOI: 10.1021/acs.analchem.9b00607 PMID: 30919620
pubmed: 30919620
doi: 10.1021/acs.analchem.9b00607
doi:

Substances chimiques

Benzoxazoles 0
Fluorescent Dyes 0
Oligopeptides 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

6669-6674

Auteurs

Ji-Woo Choi (JW)

KU-KIST Graduate School of Converging Science and Technology , Korea University , 145 Anam-ro , Seongbuk-gu, Seoul 02841 , Republic of Korea.
ORCID: 0000-0001-7325-5918

Seung Taek Hong (ST)

KU-KIST Graduate School of Converging Science and Technology , Korea University , 145 Anam-ro , Seongbuk-gu, Seoul 02841 , Republic of Korea.
ORCID: 0000-0002-2776-6087

Mun Seok Kim (MS)

Department of Chemistry , Daejin University , 1007 Hoguk-ro , Pocheon-si , Gyeonggi-do 11159 , Republic of Korea.

Kyu Cheol Paik (KC)

Department of Chemistry , Daejin University , 1007 Hoguk-ro , Pocheon-si , Gyeonggi-do 11159 , Republic of Korea.

Man So Han (MS)

Department of Chemistry , Daejin University , 1007 Hoguk-ro , Pocheon-si , Gyeonggi-do 11159 , Republic of Korea.

Bong Rae Cho (BR)

Department of Chemistry , Daejin University , 1007 Hoguk-ro , Pocheon-si , Gyeonggi-do 11159 , Republic of Korea.
Department of Chemistry , Korea University , 145 Anam-ro , Seongbuk-gu, Seoul 02841 , Republic of Korea.
ORCID: 0000-0001-8560-5399

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Two-Photon Probes for Golgi Apparatus:...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Mort cellulaire › Mort cellulaire régulée › Apoptose › Two-Photon Probes for Golgi Apparatus:...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérobicycliques › Benzoxazoles › Two-Photon Probes for Golgi Apparatus:...
questionsmedicales.fr › Techniques d'investigation › Stabilité de médicament › Two-Photon Probes for Golgi Apparatus:...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Indicateurs et réactifs › Luminescents › Colorants fluorescents › Two-Photon Probes for Golgi Apparatus:...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Appareil de Golgi › Two-Photon Probes for Golgi Apparatus:...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HeLa › Two-Photon Probes for Golgi Apparatus:...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Two-Photon Probes for Golgi Apparatus:...
questionsmedicales.fr › Techniques d'investigation › Microscopie de fluorescence › Two-Photon Probes for Golgi Apparatus:...
questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › Oligopeptides › Two-Photon Probes for Golgi Apparatus:...
questionsmedicales.fr › Phénomènes physiques › Rayonnement › Rayonnement non ionisant › Lumière › Photons › Two-Photon Probes for Golgi Apparatus:...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Rat Sprague-Dawley › Two-Photon Probes for Golgi Apparatus:...