Chemoenzymatic Total Synthesis of Deoxy-, epi-, and Podophyllotoxin and a Biocatalytic Kinetic Resolution of Dibenzylbutyrolactones.

2-oxoglutarate-dependent dioxygenases biocatalysis lignans podophyllotoxin total synthesis

Journal

Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543

Informations de publication

Date de publication:
11 06 2019
Historique:
received: 23 01 2019
pubmed: 29 3 2019
medline: 9 9 2020
entrez: 29 3 2019
Statut: ppublish

Résumé

Podophyllotoxin is probably the most prominent representative of lignan natural products. Deoxy-, epi-, and podophyllotoxin, which are all precursors to frequently used chemotherapeutic agents, were prepared by a stereodivergent biotransformation and a biocatalytic kinetic resolution of the corresponding dibenzylbutyrolactones with the same 2-oxoglutarate-dependent dioxygenase. The reaction can be conducted on 2 g scale, and the enzyme allows tailoring of the initial, "natural" structure and thus transforms various non-natural derivatives. Depending on the substitution pattern, the enzyme performs an oxidative C-C bond formation by C-H activation or hydroxylation at the benzylic position prone to ring closure.

Identifiants

pubmed: 30920120
doi: 10.1002/anie.201900926
pmc: PMC6563474
doi:

Substances chimiques

Lactones 0
Podophyllotoxin L36H50F353

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

8226-8230

Subventions

Organisme : Austrian Science Fund
ID : P 31276-B21
Pays : International

Informations de copyright

© 2019 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.

Références

Biochemistry. 2011 Mar 15;50(10):1739-54
pubmed: 21250661
Angew Chem Int Ed Engl. 2015 Apr 20;54(17):5117-21
pubmed: 25712404
J Org Chem. 2018 Jul 20;83(14):7407-7415
pubmed: 29771530
Chem Rev. 2010 Feb 10;110(2):932-48
pubmed: 19769330
Nat Chem Biol. 2013 May;9(5):285-8
pubmed: 23594772
Eur J Cancer. 1998 Sep;34(10):1514-21
pubmed: 9893622
Org Lett. 2011 May 20;13(10):2594-7
pubmed: 21488628
Nat Chem Biol. 2008 Mar;4(3):186-93
pubmed: 18277980
Biochemistry. 2011 Jul 12;50(27):6063-72
pubmed: 21648411
Angew Chem Weinheim Bergstr Ger. 2015 Apr 20;127(17):5206-5210
pubmed: 27546918
Chem Rev. 2004 Feb;104(2):939-86
pubmed: 14871146
Adv Synth Catal. 2013 Sep 16;355(13):2499-2505
pubmed: 24415960
J Bacteriol. 1997 Sep;179(18):5677-83
pubmed: 9294421
Angew Chem Int Ed Engl. 2017 Jul 24;56(31):8942-8973
pubmed: 28407390
Angew Chem Int Ed Engl. 2011 Apr 4;50(15):3362-74
pubmed: 21413105
Angew Chem Int Ed Engl. 2012 Apr 2;51(14):3428-31
pubmed: 22374809
Curr Opin Chem Biol. 2013 Aug;17(4):532-6
pubmed: 23870699
Biochem Biophys Res Commun. 2009 Dec 18;390(3):506-10
pubmed: 19850012
Chem Rev. 2014 Apr 9;114(7):3919-62
pubmed: 24400737
Acc Chem Res. 2012 Jun 19;45(6):826-39
pubmed: 22017496
Curr Opin Biotechnol. 2019 Dec;60:29-38
pubmed: 30583278
Appl Environ Microbiol. 2011 Oct;77(19):6926-30
pubmed: 21821743
Angew Chem Int Ed Engl. 2019 Jun 11;58(24):8226-8230
pubmed: 30920120
Appl Environ Microbiol. 1999 Sep;65(9):4028-31
pubmed: 10473412
Biosci Biotechnol Biochem. 2000 Apr;64(4):746-50
pubmed: 10830487
Angew Chem Int Ed Engl. 2018 Apr 23;57(18):5037-5041
pubmed: 29481729
Nature. 2019 Jan;565(7737):67-72
pubmed: 30568304
Biochem J. 1996 Jan 1;313 ( Pt 1):185-91
pubmed: 8546682
Org Lett. 2019 Jan 4;21(1):228-232
pubmed: 30550285
Org Lett. 2018 Mar 16;20(6):1651-1654
pubmed: 29508607
Chem Soc Rev. 2011 Sep;40(9):4740-61
pubmed: 21666903
Science. 2015 Sep 11;349(6253):1224-8
pubmed: 26359402
Enzyme Microb Technol. 1993 Dec;15(12):1051-6
pubmed: 7505594
Appl Microbiol Biotechnol. 2010 Oct;88(3):719-26
pubmed: 20665018
Chem Pharm Bull (Tokyo). 2003 Jul;51(7):848-50
pubmed: 12843593
Chemistry. 2017 Sep 7;23(50):12040-12063
pubmed: 28514518
Appl Microbiol Biotechnol. 2011 Mar;89(6):1929-38
pubmed: 21069315
J Inorg Biochem. 2006 Apr;100(4):644-69
pubmed: 16513174
Appl Environ Microbiol. 1996 Jun;62(6):1903-7
pubmed: 16535329
Appl Microbiol Biotechnol. 2014 May;98(9):3869-76
pubmed: 24682483
Angew Chem Int Ed Engl. 2014 May 5;53(19):4750-2
pubmed: 24711286
J Biol Chem. 2013 Jun 14;288(24):17065-73
pubmed: 23632016
Org Biomol Chem. 2009 Oct 7;7(19):4082-7
pubmed: 19763315
J Am Chem Soc. 2008 Oct 29;130(43):14022-3
pubmed: 18828590
Proc Natl Acad Sci U S A. 2005 Jul 19;102(29):10111-6
pubmed: 16002467
Chemistry. 2017 Apr 3;23(19):4467-4526
pubmed: 27726183
J Org Chem. 2019 May 3;84(9):5831-5837
pubmed: 30920215
Biochim Biophys Acta. 2016 May;1864(5):453-70
pubmed: 26845569
J Am Chem Soc. 2018 Jan 24;140(3):1165-1169
pubmed: 29283572

Auteurs

Mattia Lazzarotto (M)

Institute of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28/II, 8010, Graz, Austria.

Lucas Hammerer (L)

Institute of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28/II, 8010, Graz, Austria.
Austrian Centre of Industrial Biotechnology, c/o University of Graz, Graz, Austria.

Michael Hetmann (M)

Institute of Molecular Biosciences, University of Graz, Humboldtstraße 50/III, 8010, Graz, Austria.

Annika Borg (A)

Institute of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28/II, 8010, Graz, Austria.

Luca Schmermund (L)

Institute of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28/II, 8010, Graz, Austria.

Lorenz Steiner (L)

Institute of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28/II, 8010, Graz, Austria.

Peter Hartmann (P)

Institute of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28/II, 8010, Graz, Austria.

Ferdinand Belaj (F)

Institute of Chemistry, Inorganic Chemistry, University of Graz, Schubertstraße 1/III, 8010, Graz, Austria.

Wolfgang Kroutil (W)

Institute of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28/II, 8010, Graz, Austria.

Karl Gruber (K)

Institute of Molecular Biosciences, University of Graz, Humboldtstraße 50/III, 8010, Graz, Austria.

Michael Fuchs (M)

Institute of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28/II, 8010, Graz, Austria.

Articles similaires

Saccharomyces cerevisiae Aldehydes Biotransformation Flavoring Agents Lipoxygenase
Quorum Sensing Cystic Fibrosis Mucus Pseudomonas aeruginosa Humans
Streptomyces Antifungal Agents Macrolides Lactones Polyketide Synthases
Gossypium Lactones Droughts Gene Expression Regulation, Plant Reactive Oxygen Species

Classifications MeSH