Establishment of the molecular beacon-loop-mediated isothermal amplification method for the rapid detection of Porphyromonas gingivalis.
Loop-mediated isothermal amplification (LAMP)
Molecular diagnosis
Porphyromonas
Rapid detection
Journal
Journal of microbiological methods
ISSN: 1872-8359
Titre abrégé: J Microbiol Methods
Pays: Netherlands
ID NLM: 8306883
Informations de publication
Date de publication:
05 2019
05 2019
Historique:
received:
05
11
2018
revised:
22
01
2019
accepted:
22
01
2019
pubmed:
30
3
2019
medline:
11
6
2020
entrez:
30
3
2019
Statut:
ppublish
Résumé
Porphyromonas gingivalis, a clinically important oral pathogen causing periodontal disease, is difficult to culture in routine conditions. Hence, it is necessary to establish a reliable technique to detect this pathogen. Previously, our laboratory developed a new isothermal detection method, called MB-LAMP (molecular beacon-Loop-mediated isothermal amplification), which combines the advantages of LAMP and qPCR through the accurate and quantitative detection of LAMP products. This approach offers significant potential for the point-of-care detection of P. gingivalis. Here, MB-LAMP was used to detect P. gingivalis targeting a specific fragment, and the sensitivity was as high as 1.4 × 10
Identifiants
pubmed: 30922631
pii: S0167-7012(18)30883-2
doi: 10.1016/j.mimet.2019.01.013
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
68-72Informations de copyright
Copyright © 2019. Published by Elsevier B.V.