Combinatorial Cytotoxic Effects of Gelam Honey and 5-Fluorouracil against Human Adenocarcinoma Colon Cancer HT-29 Cells In Vitro.
Journal
International journal of cell biology
ISSN: 1687-8876
Titre abrégé: Int J Cell Biol
Pays: United States
ID NLM: 101517861
Informations de publication
Date de publication:
2019
2019
Historique:
received:
11
11
2018
revised:
09
01
2019
accepted:
17
01
2019
entrez:
30
3
2019
pubmed:
30
3
2019
medline:
30
3
2019
Statut:
epublish
Résumé
Combination of natural products with chemodrugs is becoming a trend in discovering new therapeutics approach for enhancing the cancer treatment process. In the present study, we aimed to investigate the cytotoxic and apoptosis induction of Gelam honey (GH) combined with or without 5-Fluorouracil (5-FU) on HT-29 cells. The cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay to assess cytotoxicity. Morphological changes and apoptosis were determined by the inverted microscope, Annexin V-FITC, and DNA fragmentation via flow cytometric analysis, respectively. Our results demonstrate that combined treatment revealed a remarkable and concentration-dependent cytotoxic effect on HT-29 cells in comparison with GH and 5-FU alone. Flow cytometry analysis showed that early apoptosis event was more pronounced in combined treatment. In addition, compared to 5-FU alone, apoptosis of HT-29 cells treated with combinations of GH and 5-FU demonstrated increasing percentages of fragmented DNA. Our results suggest that GH has a synergistic cytotoxic effect with 5-FU in HT-29 cell lines
Identifiants
pubmed: 30923553
doi: 10.1155/2019/3059687
pmc: PMC6408991
doi:
Types de publication
Journal Article
Langues
eng
Pagination
3059687Commentaires et corrections
Type : ErratumIn
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