SCOTfluors: Small, Conjugatable, Orthogonal, and Tunable Fluorophores for In Vivo Imaging of Cell Metabolism.


Journal

Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543

Informations de publication

Date de publication:
20 05 2019
Historique:
received: 12 01 2019
pubmed: 30 3 2019
medline: 9 9 2020
entrez: 30 3 2019
Statut: ppublish

Résumé

The transport and trafficking of metabolites are critical for the correct functioning of live cells. However, in situ metabolic imaging studies are hampered by the lack of fluorescent chemical structures that allow direct monitoring of small metabolites under physiological conditions with high spatial and temporal resolution. Herein, we describe SCOTfluors as novel small-sized multi-colored fluorophores for real-time tracking of essential metabolites in live cells and in vivo and for the acquisition of metabolic profiles from human cancer cells of variable origin.

Identifiants

pubmed: 30924239
doi: 10.1002/anie.201900465
pmc: PMC6563150
doi:

Substances chimiques

Fluorescent Dyes 0
Ionophores 0
Green Fluorescent Proteins 147336-22-9

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

6911-6915

Subventions

Organisme : Wellcome Trust
ID : 100104/Z/12/Z
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M025160/1
Pays : United Kingdom
Organisme : OPTIMA
ID : EP/L016559/1
Pays : International
Organisme : MSCA Fellowship
ID : 704912
Pays : International
Organisme : Medical Research Scotland
ID : 879-2015
Pays : International
Organisme : Royal Society
ID : IEC\R3\170132
Pays : International
Organisme : Ministerio de Economía, Industria y Competitividad, Gobierno de España
ID : CTQ2017-85378-R
Pays : International
Organisme : European Research Council
ID : 771443
Pays : International

Informations de copyright

© 2019 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.

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Auteurs

Sam Benson (S)

Centre for Inflammation Research, The University of Edinburgh, EH16 4TJ, Edinburgh, UK.

Antonio Fernandez (A)

Centre for Inflammation Research, The University of Edinburgh, EH16 4TJ, Edinburgh, UK.

Nicole D Barth (ND)

Centre for Inflammation Research, The University of Edinburgh, EH16 4TJ, Edinburgh, UK.

Fabio de Moliner (F)

Centre for Inflammation Research, The University of Edinburgh, EH16 4TJ, Edinburgh, UK.

Mathew H Horrocks (MH)

UK Dementia Research Institute and EaStCHEM School of Chemistry, The University of Edinburgh, EH9 3FJ, Edinburgh, UK.

C Simon Herrington (CS)

Edinburgh Cancer Research Centre, EH4 2XR, Edinburgh, UK.

Jose Luis Abad (JL)

Research Unit on Bioactive Molecules, Institute for Advanced Chemistry of Catalonia, 08034, Barcelona, Spain.
University of Barcelona, Faculty of Pharmacy, Unit of Pharmaceutical Chemistry (CSIC Associated Unit), Barcelona, Spain.

Antonio Delgado (A)

Research Unit on Bioactive Molecules, Institute for Advanced Chemistry of Catalonia, 08034, Barcelona, Spain.
University of Barcelona, Faculty of Pharmacy, Unit of Pharmaceutical Chemistry (CSIC Associated Unit), Barcelona, Spain.

Lisa Kelly (L)

Centre for Inflammation Research, The University of Edinburgh, EH16 4TJ, Edinburgh, UK.

Ziyuan Chang (Z)

Centre for Inflammation Research, The University of Edinburgh, EH16 4TJ, Edinburgh, UK.

Yi Feng (Y)

Centre for Inflammation Research, The University of Edinburgh, EH16 4TJ, Edinburgh, UK.

Miyako Nishiura (M)

Graduate School of Engineering, Osaka University, Suita, Japan.

Yuichiro Hori (Y)

Graduate School of Engineering, Osaka University, Suita, Japan.

Kazuya Kikuchi (K)

Graduate School of Engineering, Osaka University, Suita, Japan.

Marc Vendrell (M)

Centre for Inflammation Research, The University of Edinburgh, EH16 4TJ, Edinburgh, UK.

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Classifications MeSH