Retrograde perfusion in isolated perfused mouse lungs-Feasibility and effects on cytokine levels and pulmonary oedema formation.
Acute Lung Injury
/ diagnosis
Animals
Biomarkers
/ analysis
Bronchoalveolar Lavage Fluid
/ chemistry
Cytokines
/ analysis
Disease Models, Animal
Edema
/ diagnosis
Ephrin-B2
/ analysis
Feasibility Studies
Female
Humans
In Vitro Techniques
/ methods
Lung
/ immunology
Mice
Perfusion
/ adverse effects
Receptor, EphB4
/ analysis
Respiration, Artificial
/ adverse effects
Time Factors
Up-Regulation
ephB4
ephrinB2
isolated perfused lungs
oedema formation
retrograde perfusion
Journal
Basic & clinical pharmacology & toxicology
ISSN: 1742-7843
Titre abrégé: Basic Clin Pharmacol Toxicol
Pays: England
ID NLM: 101208422
Informations de publication
Date de publication:
Sep 2019
Sep 2019
Historique:
received:
12
10
2018
accepted:
22
03
2019
pubmed:
30
3
2019
medline:
15
2
2020
entrez:
30
3
2019
Statut:
ppublish
Résumé
Retrograde lung vascular perfusion can appear in high-risk surgeries. The present report is the first to study long-term retrograde perfusion of isolated perfused mouse lungs (IPLs) and to use the tyrosine kinase ephB4 and its ligand ephrinB2 as potential markers for acute lung injury. Mouse lungs were subjected to anterograde or retrograde perfusion with normal-pressure ventilation (NV) or high-pressure ventilation (=overventilation, OV) for 4 hours. Outcome parameters were cytokine, ephrinB2 and ephB4 levels in perfusate samples and bronchoalveolar lavage (BAL), and the wet-to-dry ratio. Anterograde perfusion was feasible for 4 hours, while lungs receiving retrograde perfusion presented considerable collapse rates. Retrograde perfusion resulted in an increased wet-to-dry ratio when combined with high-pressure ventilation; other physiological parameters were not affected. Cytokine levels in BAL and perfusate, as well as levels of soluble ephB4 in BAL were increased in OV, while soluble ephrinB2 BAL levels were increased in retrograde perfusion. BAL levels of ephrinB2 and ephB4 were also determined in vivo, including mice ventilated for 7 hours with normal-volume ventilation (NVV) or high-volume ventilation (HVV) with increased levels of ephB4 in HVV BAL compared to NVV. Retrograde perfusion in IPL is limited as a routine method to investigate effects due to collapse for yet unclear reasons. If successful, retrograde perfusion has an influence on pulmonary oedema formation. In BAL, ephrinB2 seems to be up-regulated by flow reversal, while ephB4 is a marker for acute lung injury.
Substances chimiques
Biomarkers
0
Cytokines
0
EFNB2 protein, mouse
0
Ephrin-B2
0
Ephb4 protein, mouse
EC 2.7.10.1
Receptor, EphB4
EC 2.7.10.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
279-288Subventions
Organisme : RWTH Aachen University
ID : 114/16
Informations de copyright
© 2019 Nordic Association for the Publication of BCPT (former Nordic Pharmacological Society).
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