Amorphous Silica Nanoparticles Obtained by Laser Ablation Induce Inflammatory Response in Human Lung Fibroblasts.
MRC-5 cell line
inflammatory response
oxidative stress
silica nanoparticles
Journal
Materials (Basel, Switzerland)
ISSN: 1996-1944
Titre abrégé: Materials (Basel)
Pays: Switzerland
ID NLM: 101555929
Informations de publication
Date de publication:
28 Mar 2019
28 Mar 2019
Historique:
received:
20
02
2019
revised:
22
03
2019
accepted:
25
03
2019
entrez:
31
3
2019
pubmed:
31
3
2019
medline:
31
3
2019
Statut:
epublish
Résumé
Silica nanoparticles (SiO₂ NPs) represent environmentally born nanomaterials that are used in multiple biomedical applications. Our aim was to study the amorphous SiO₂ NP-induced inflammatory response in MRC-5 human lung fibroblasts up to 72 hours of exposure. The intracellular distribution of SiO₂ NPs was measured by transmission electron microscopy (TEM). The lactate dehydrogenase (LDH) test was used for cellular viability evaluation. We have also investigated the lysosomes formation, protein expression of interleukins (IL-1β, IL-2, IL-6, IL-8, and IL-18), COX-2, Nrf2, TNF-α, and nitric oxide (NO) production. Our results showed that the level of lysosomes increased in time after exposure to the SiO₂ NPs. The expressions of interleukins and COX-2 were upregulated, whereas the expressions and activities of MMP-2 and MMP-9 decreased in a time-dependent manner. Our findings demonstrated that the exposure of MRC-5 cells to 62.5 µg/mL of SiO₂ NPs induced an inflammatory response.
Identifiants
pubmed: 30925685
pii: ma12071026
doi: 10.3390/ma12071026
pmc: PMC6479987
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Unitatea Executiva pentru Finantarea Invatamantului Superior, a Cercetarii, Dezvoltarii si Inovarii
ID : 13/2015/SIINN ERA-NET
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