A novel linear 3-O-methylated galactan isolated from Cantharellus cibarius activates macrophages.
Agaricales
/ chemistry
Animals
Fruiting Bodies, Fungal
/ chemistry
Galactans
/ chemistry
Gene Knockdown Techniques
Interleukin-1beta
/ metabolism
Interleukin-6
/ metabolism
MAP Kinase Signaling System
/ drug effects
Macrophage Activation
/ drug effects
Macrophages
/ drug effects
Mice
Molecular Structure
Molecular Weight
Nitric Oxide
/ metabolism
Phagocytosis
/ drug effects
RAW 264.7 Cells
Structure-Activity Relationship
Toll-Like Receptor 2
/ genetics
Tumor Necrosis Factor-alpha
/ metabolism
3-O-methylated galactan
Cantharellus cibarius
Macrophage
Polysaccharide
TLR2
Journal
Carbohydrate polymers
ISSN: 1879-1344
Titre abrégé: Carbohydr Polym
Pays: England
ID NLM: 8307156
Informations de publication
Date de publication:
15 Jun 2019
15 Jun 2019
Historique:
received:
11
12
2018
revised:
18
02
2019
accepted:
03
03
2019
entrez:
31
3
2019
pubmed:
31
3
2019
medline:
7
8
2019
Statut:
ppublish
Résumé
A novel polysaccharide (WCCP-N-b) with a molecular weight of 18 kDa was isolated and purified from the fruiting bodies of Cantharellus cibarius. Monosaccharide composition, methylation analysis and NMR spectra indicated that WCCP-N-b was a linear α-1,6-galactan, partially methylated at O-3 of galactose. The molar ratio of Gal, 3-methylated-Gal, Glc and Man was 14.4:4.6:1.0:1.2. WCCP-N-b could significantly increase macrophage phagocytosis, release of NO and secretion of TNF-α, IL-6 and IL-1β. On a cellular mechanistic level, WCCP-N-b activated MAPKs and NF-κB signaling pathway via Toll-like receptor 2 (TLR2). To further elucidate the structure-function relationship, WCCP-N-b was hydrolyzed by acid. Four degraded fragments were obtained, with molecular weights of 16.1 kDa, 11.2 kDa, 5 kDa and 3.5 kDa, respectively. Their macrophage activation effects were significantly decreased along with the molecular weight decrease. Collectively, WCCP-N-b could activate RAW264.7 cells, and the activation effect was related to its molecular weight.
Identifiants
pubmed: 30926005
pii: S0144-8617(19)30268-1
doi: 10.1016/j.carbpol.2019.03.002
pii:
doi:
Substances chimiques
Galactans
0
Interleukin-1beta
0
Interleukin-6
0
TLR2 protein, human
0
Toll-Like Receptor 2
0
Tumor Necrosis Factor-alpha
0
interleukin-6, mouse
0
Nitric Oxide
31C4KY9ESH
Types de publication
Journal Article
Langues
eng
Pagination
34-43Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.