Biofilm development in L. fermentum under shear flow & sequential GIT digestion.
Lactobacillus fermentum; biofilm
in vitro digestion
BioFlux 1000Z
flow
simulation
Journal
FEMS microbiology letters
ISSN: 1574-6968
Titre abrégé: FEMS Microbiol Lett
Pays: England
ID NLM: 7705721
Informations de publication
Date de publication:
01 03 2019
01 03 2019
Historique:
received:
16
10
2018
accepted:
29
03
2019
pubmed:
31
3
2019
medline:
25
2
2020
entrez:
31
3
2019
Statut:
ppublish
Résumé
The objective of this study was to investigate biofilm formation by Lactobacillus fermentum under physiologically relevant shear conditions both in the presence and absence of a food matrix and under simulated conditions of digestion. This was done using batch and flow biofilms of L. fermentum strains under conditions simulating digestion in the human gastrointestinal tract and shear flow using a high throughput platform BioFlux 1000Z system. The putative probiotic strain, PL29, was found to be capable of adhesion and biofilm formation in mucin-coated microfluidic channels under liquid flow conditions mimicking those of the GIT. Based on these in vitro measurements, we conclude that L. fermentum strain PL29 could be an effective probiotic for human consumption.
Identifiants
pubmed: 30927413
pii: 5423880
doi: 10.1093/femsle/fnz064
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Commentaires et corrections
Type : ErratumIn
Informations de copyright
© FEMS 2019.