Protein Expression Analysis by Western Blot and Protein-Protein Interactions.
Monoclonal antibody
Pneumolysin
PpmA
Urine
Western-blot
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
1
4
2019
pubmed:
1
4
2019
medline:
6
8
2019
Statut:
ppublish
Résumé
Western blot analysis is widely used for detecting protein expression, analysis of protein-protein interactions, and searching for new biomarkers. Also, it is a diagnostic tool used for detection of human diseases and microorganism infections.Some Streptococcus pneumoniae proteins are important virulence factors and a few of them are diagnostic markers. Here, we describe the detection of two pneumococcal proteins, pneumolysin and PpmA, in human urine by using monoclonal and polyclonal antibodies.
Identifiants
pubmed: 30929209
doi: 10.1007/978-1-4939-9199-0_9
doi:
Substances chimiques
Antibodies, Bacterial
0
Antibodies, Monoclonal
0
Bacterial Proteins
0
PpmA protein, Streptococcus pneumoniae
0
Streptolysins
0
plY protein, Streptococcus pneumoniae
0
Types de publication
Journal Article
Langues
eng