Potential function of TGF-β isoforms in maturation-stage ameloblasts.

Ameloblasts Amelogenesis Amelogenin Animals Matrix Metalloproteinase 20 Mice Protein Isoforms Proteins Transforming Growth Factor beta1 Transforming Growth Factor beta3
Ameloblast Apoptosis Enamel Endocytosis Transforming growth factor-beta

Journal

Journal of oral biosciences
ISSN: 1880-3865
Titre abrégé: J Oral Biosci
Pays: Netherlands
ID NLM: 101226721

Informations de publication

Date de publication:
03 2019
Historique:
received: 19 11 2018
revised: 18 12 2018
accepted: 19 12 2018
entrez: 2 4 2019
pubmed: 2 4 2019
medline: 28 3 2020
Statut: ppublish

Résumé

To investigate potential functions of transforming growth factor-beta (TGF-β) isoforms in maturation-stage ameloblasts during amelogenesis. In vivo activation of TGF-β was characterized by using matrix metalloproteinase 20 null (Mmp20 TGF-β1 and TGF-β3 were present in the enamel matrix of developing teeth which were activated by MMP20 in vivo. A genetic study revealed that the three TGF-β isoforms upregulate kallikrein 4 (KLK4) mRNA levels but downregulate carbonic anhydrase II. Moreover, TGF-β1 and TGF-β2 significantly upregulated the mRNA level of amelotin, whereas TGF-β3 dramatically downregulated the mRNA levels of odontogenic ameloblast-associated protein (ODAM), family with sequence similarity 83 member H (FAM83H), and alkaline phosphatase (ALP). Immunostaining analysis showed that the apoptosis of mHAT9d cells is induced by three TGF-β isoforms, with TGF-β3 being most effective. Both TGF-β1 and TGF-β3 induced endocytosis of amelogenin. We propose that TGF-β is regulated in an isoform-specific manner to perform multiple biological functions such as gene expression related to the structure of basal lamina/ameloblasts, mineral ion transport, apoptosis, and endocytosis in maturation-stage ameloblasts.

Identifiants

DOI: 10.1016/j.job.2018.12.002 PMID: 30929801
pubmed: 30929801
pii: S1349-0079(18)30155-5
doi: 10.1016/j.job.2018.12.002
pii:
doi:

Substances chimiques

Amelogenin 0
Protein Isoforms 0
Proteins 0
Transforming Growth Factor beta1 0
Transforming Growth Factor beta3 0
Matrix Metalloproteinase 20 EC 3.4.24.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

43-54

Informations de copyright

Copyright © 2019 Japanese Association for Oral Biology. Published by Elsevier B.V. All rights reserved.

Auteurs

Miu Okubo (M)

Department of Periodontology, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan. Electronic address: okubo-miu@tsurumi-u.ac.jp.

Risako Chiba (R)

Department of Biochemistry and Molecular Biology, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan. Electronic address: chiba-r@tsurumi-u.ac.jp.

Takeo Karakida (T)

Department of Biochemistry and Molecular Biology, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan. Electronic address: karakida-t@tsurumi-u.ac.jp.

Hajime Yamazaki (H)

The Forsyth Institute, 245 First Street, Cambridge, Boston, MA 02142, USA; Department of Development Biology, Harvard School of Dental Medicine, 188 Longwood Avenue, Boston, MA 02115, USA.

Ryuji Yamamoto (R)

Department of Biochemistry and Molecular Biology, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan. Electronic address: yamamoto-rj@tsurumi-u.ac.jp.

Saeko Kobayashi (S)

Department of Pediatric Dentistry, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan. Electronic address: kinoshita-saeko@tsurumi-u.ac.jp.

Takahiko Niwa (T)

Department of Periodontology, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan. Electronic address: niwa-takahiko@tsurumi-u.ac.jp.

Henry C Margolis (HC)

The Forsyth Institute, 245 First Street, Cambridge, Boston, MA 02142, USA; Department of Development Biology, Harvard School of Dental Medicine, 188 Longwood Avenue, Boston, MA 02115, USA.

Takatoshi Nagano (T)

Department of Periodontology, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan. Electronic address: nagano-takatoshi@tsurumi-u.ac.jp.

Yasuo Yamakoshi (Y)

Department of Biochemistry and Molecular Biology, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan. Electronic address: yamakoshi-y@tsurumi-u.ac.jp.

Kazuhiro Gomi (K)

Department of Periodontology, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan. Electronic address: gomi-k@tsurumi-u.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Cellules › Cellules épithéliales › Améloblastes › Potential function of TGF-β isoforms in...
questionsmedicales.fr › Phénomènes physiologiques › Croissance et développement › Morphogenèse › Odontogenèse › Amélogenèse › Potential function of TGF-β isoforms in...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de l'émail dentaire › Amélogénine › Potential function of TGF-β isoforms in...
questionsmedicales.fr › Eucaryotes › Animaux › Potential function of TGF-β isoforms in...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Secreted matrix metalloproteinases › Énamelysine › Potential function of TGF-β isoforms in...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Potential function of TGF-β isoforms in...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Isoformes de protéines › Potential function of TGF-β isoforms in...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Potential function of TGF-β isoforms in...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Protéines de la superfamille du TGF bêta › Facteur de croissance transformant bêta › Facteur de croissance transformant bêta-1 › Potential function of TGF-β isoforms in...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Protéines de la superfamille du TGF bêta › Facteur de croissance transformant bêta › Facteur de croissance transformant bêta-3 › Potential function of TGF-β isoforms in...