Neutrophil Extracellular Traps Confine Pseudomonas aeruginosa Ocular Biofilms and Restrict Brain Invasion.
P. aeruginosa
biofilms
cornea
intravital microscopy
keratitis
neutrophil extracellular traps
Journal
Cell host & microbe
ISSN: 1934-6069
Titre abrégé: Cell Host Microbe
Pays: United States
ID NLM: 101302316
Informations de publication
Date de publication:
10 Apr 2019
10 Apr 2019
Historique:
received:
07
10
2018
revised:
30
11
2018
accepted:
21
02
2019
pubmed:
2
4
2019
medline:
22
11
2019
entrez:
2
4
2019
Statut:
ppublish
Résumé
Bacterial biofilm infections are difficult to eradicate because of antibiotic insusceptibility and high recurrence rates. Biofilm formation by Pseudomonas aeruginosa, a leading cause of bacterial keratitis, is facilitated by the bacterial Psl exopolysaccharide and associated with heightened virulence. Using intravital microscopy, we observed that neutrophilic recruitment to corneal infections limits P. aeruginosa biofilms to the outer eye surface, preventing bacterial dissemination. Neutrophils moved to the base of forming biofilms, where they underwent neutrophil extracellular trap formation (NETosis) in response to high expression of the bacterial type-3 secretion system (T3SS). NETs formed a barrier "dead zone," confining bacteria to the external corneal environment and inhibiting bacterial dissemination into the brain. Once formed, ocular biofilms were resistant to antibiotics and neutrophil killing, advancing eye pathology. However, blocking both Psl and T3SS together with antibiotic treatment broke down the biofilm and reversed keratitis, suggesting future therapeutic strategies for this intractable infection.
Identifiants
pubmed: 30930127
pii: S1931-3128(19)30103-9
doi: 10.1016/j.chom.2019.02.007
pmc: PMC7364305
mid: NIHMS1600895
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
526-536.e4Subventions
Organisme : NEI NIH HHS
ID : R01 EY022054
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.
Références
Microb Ecol. 2014 Jul;68(1):1-12
pubmed: 24096885
PLoS Pathog. 2014 Nov 06;10(11):e1004479
pubmed: 25375398
Nat Rev Immunol. 2018 Feb;18(2):134-147
pubmed: 28990587
Exp Eye Res. 2014 Mar;120:61-70
pubmed: 24462632
J Clin Invest. 2017 Jun 1;127(6):2249-2261
pubmed: 28463232
Br J Ophthalmol. 2003 Apr;87(4):512-4
pubmed: 12642331
Microbiol Mol Biol Rev. 2014 Sep;78(3):510-43
pubmed: 25184564
Nat Immunol. 2014 Nov;15(11):1017-25
pubmed: 25217981
Front Immunol. 2016 Aug 12;7:302
pubmed: 27570525
Pathogens. 2014 Aug 18;3(3):680-703
pubmed: 25438018
Pathog Dis. 2013 Apr;67(3):159-73
pubmed: 23620179
FEMS Microbiol Rev. 2012 Jul;36(4):893-916
pubmed: 22212072
J Exp Med. 2012 Jul 2;209(7):1273-87
pubmed: 22734046
Invest Ophthalmol Vis Sci. 2012 Jan 25;53(1):267-72
pubmed: 22159005
Antimicrob Agents Chemother. 2010 Oct;54(10):4409-15
pubmed: 20696878
Methods Enzymol. 2001;336:144-51
pubmed: 11398395
Cell Microbiol. 2012 Jan;14(1):95-106
pubmed: 21951860
Mucosal Immunol. 2017 Sep;10(5):1145-1159
pubmed: 28120849
mBio. 2014 Jun 10;5(3):e01080-14
pubmed: 24917597
J Bacteriol. 2006 Dec;188(23):8213-21
pubmed: 16980452
Antimicrob Agents Chemother. 2014 Aug;58(8):4384-91
pubmed: 24841258
mBio. 2017 Jun 20;8(3):
pubmed: 28634237
Sci Rep. 2018 Sep 19;8(1):14047
pubmed: 30232373
J Cell Biol. 2010 Nov 1;191(3):677-91
pubmed: 20974816
Am J Clin Pathol. 1973 Aug;60(2):234-40
pubmed: 4124318
J Clin Microbiol. 2005 Oct;43(10):5085-90
pubmed: 16207967
Nat Methods. 2015 May;12(5):445-52
pubmed: 25775045
Arch Ophthalmol. 1991 Apr;109(4):503-5
pubmed: 2012549
Sci Rep. 2017 Nov 22;7(1):16065
pubmed: 29167572
Cont Lens Anterior Eye. 2007 May;30(2):94-107
pubmed: 17084658
Prog Retin Eye Res. 2012 May;31(3):271-85
pubmed: 22426080
Nat Immunol. 2010 Apr;11(4):295-302
pubmed: 20228796
Nature. 2013 Jun 20;498(7454):371-5
pubmed: 23708969
Proc Natl Acad Sci U S A. 2018 Jul 10;115(28):7416-7421
pubmed: 29941565
Nat Med. 2012 Sep;18(9):1386-93
pubmed: 22922410
J Immunol Res. 2016;2016:1469780
pubmed: 27006954
Front Immunol. 2012 Sep 21;3:296
pubmed: 23049533
Toxins (Basel). 2018 May 04;10(5):
pubmed: 29734720
Sci Rep. 2018 Jun 20;8(1):9637
pubmed: 29925842
Infect Immun. 2011 Feb;79(2):595-605
pubmed: 21115716
Am J Pathol. 2006 Jul;169(1):294-302
pubmed: 16816381
J Immunol. 2012 Feb 15;188(4):1884-95
pubmed: 22250085
PLoS Pathog. 2009 Mar;5(3):e1000354
pubmed: 19325879
Infect Immun. 2010 Nov;78(11):4500-10
pubmed: 20732998
Cell Host Microbe. 2017 May 10;21(5):611-618.e5
pubmed: 28494242
Science. 2010 Oct 15;330(6002):362-6
pubmed: 20947763
Invest Ophthalmol Vis Sci. 1987 Dec;28(12):1978-85
pubmed: 3119512
Curr Eye Res. 1987 Jan;6(1):59-62
pubmed: 3030654
Yale J Biol Med. 2016 Sep 30;89(3):325-330
pubmed: 27698616
J Theor Biol. 2012 Sep 7;308:68-78
pubmed: 22677397
Ocul Surf. 2019 Jan;17(1):119-133
pubmed: 30439473
Sci Rep. 2017 Aug 29;7(1):9880
pubmed: 28852195
Exp Eye Res. 2016 Oct;151:160-70
pubmed: 27544307
FEMS Immunol Med Microbiol. 2012 Jul;65(2):377-80
pubmed: 22309106
Sci Transl Med. 2014 Nov 12;6(262):262ra155
pubmed: 25391481
Ocul Surf. 2015 Oct;13(4):331-45
pubmed: 26220579
Front Immunol. 2016 Aug 26;7:325
pubmed: 27617014