The in vivo timeline of differentiation of engrafted human neural progenitor cells.
Bioluminescence imaging
Cerebral cortex
In vivo brain imaging
Nude mice
Stem cell differentiation
Stem cell implantation
Journal
Stem cell research
ISSN: 1876-7753
Titre abrégé: Stem Cell Res
Pays: England
ID NLM: 101316957
Informations de publication
Date de publication:
05 2019
05 2019
Historique:
received:
08
01
2019
revised:
18
03
2019
accepted:
22
03
2019
pubmed:
2
4
2019
medline:
12
3
2020
entrez:
2
4
2019
Statut:
ppublish
Résumé
Understanding the individual timeline of stem cell differentiation in vivo is critical for evaluating stem cell properties in animal models. However, with conventional ex vivo techniques, such as histology, the individual timeline of differentiation is not accessible. Therefore, we designed lentiviral plasmids with cell-specific promoters to control the expression of bioluminescence and fluorescence imaging reporters. Promoter-dependent reporter expression in transduced human induced pluripotent stem cell-derived neural progenitor cells (hNPCs) was an effective indicator of differentiation in cell culture. A 12-week in vivo imaging observation period revealed the time profile of differentiation of engrafted hNPCs in the mouse brain into astrocytes and mature neurons which was verified by immunostainings, patch-clamp electrophysiology, and light-sheet fluorescence microscopy. The lentiviral vectors validated in this study provide an efficient imaging toolbox for non-invasive and longitudinal characterization of stem cell differentiation, in vitro screenings, and in vivo studies of cell therapy in animal models.
Identifiants
pubmed: 30933718
pii: S1873-5061(19)30059-5
doi: 10.1016/j.scr.2019.101429
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101429Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.