Recurrent SLC1A2 variants cause epilepsy via a dominant negative mechanism.


Journal

Annals of neurology
ISSN: 1531-8249
Titre abrégé: Ann Neurol
Pays: United States
ID NLM: 7707449

Informations de publication

Date de publication:
06 2019
Historique:
received: 04 12 2018
revised: 11 02 2019
accepted: 31 03 2019
pubmed: 3 4 2019
medline: 31 3 2020
entrez: 3 4 2019
Statut: ppublish

Résumé

SLC1A2 is a trimeric transporter essential for clearing glutamate from neuronal synapses. Recurrent de novo SLC1A2 missense variants cause a severe, early onset developmental and epileptic encephalopathy via an unclear mechanism. We demonstrate that all 3 variants implicated in this condition localize to the trimerization domain of SLC1A2, and that the Leu85Pro variant acts via a dominant negative mechanism to reduce, but not eliminate, wild-type SLC1A2 protein localization and function. Finally, we demonstrate that treatment of a 20-month-old SLC1A2-related epilepsy patient with the SLC1A2-modulating agent ceftriaxone did not result in a significant change in daily spasm count. ANN NEUROL 2019;85:921-926.

Identifiants

pubmed: 30937933
doi: 10.1002/ana.25477
pmc: PMC6800210
mid: NIHMS1054668
doi:

Substances chimiques

Excitatory Amino Acid Transporter 2 0
SLC1A2 protein, human 0
Ceftriaxone 75J73V1629

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

921-926

Subventions

Organisme : NINDS NIH HHS
ID : R01 NS066019
Pays : United States
Organisme : NIMH NIH HHS
ID : R21 MH104318
Pays : United States
Organisme : Swiss National Science Foundation
ID : 31003A_156376
Pays : Switzerland
Organisme : Marie Curie Actions International Fellowship Program TransCure
Pays : International
Organisme : NIGMS NIH HHS
ID : T32 GM007748
Pays : United States

Informations de copyright

© 2019 American Neurological Association.

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Auteurs

Andrew B Stergachis (AB)

Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Boston, MA.

Jonai Pujol-Giménez (J)

Department of Nephrology and Hypertension, University Hospital Bern, Inselspital, Bern, Switzerland.
Institute of Biochemistry and Molecular Medicine, University of Bern, Bern, Switzerland.

Gergely Gyimesi (G)

Department of Nephrology and Hypertension, University Hospital Bern, Inselspital, Bern, Switzerland.
Institute of Biochemistry and Molecular Medicine, University of Bern, Bern, Switzerland.

Daniel Fuster (D)

Department of Nephrology and Hypertension, University Hospital Bern, Inselspital, Bern, Switzerland.
Institute of Biochemistry and Molecular Medicine, University of Bern, Bern, Switzerland.

Giusppe Albano (G)

Department of Nephrology and Hypertension, University Hospital Bern, Inselspital, Bern, Switzerland.
Institute of Biochemistry and Molecular Medicine, University of Bern, Bern, Switzerland.

Marina Troxler (M)

Department of Nephrology and Hypertension, University Hospital Bern, Inselspital, Bern, Switzerland.
Institute of Biochemistry and Molecular Medicine, University of Bern, Bern, Switzerland.

Jonathan Picker (J)

Division of Genetics and Genomics, Boston Children's Hospital, Harvard Medical School, Boston, MA.

Paul A Rosenberg (PA)

Department of Neurology, Boston Children's Hospital, Harvard Medical School, Boston, MA.

Ann Bergin (A)

Department of Neurology, Boston Children's Hospital, Harvard Medical School, Boston, MA.

Jurriaan Peters (J)

Department of Neurology, Boston Children's Hospital, Harvard Medical School, Boston, MA.

Christelle Moufawad El Achkar (CM)

Department of Neurology, Boston Children's Hospital, Harvard Medical School, Boston, MA.

Chellamani Harini (C)

Department of Neurology, Boston Children's Hospital, Harvard Medical School, Boston, MA.

Shannon Manzi (S)

Department of Pharmacy, Boston Children's Hospital, Harvard Medical School, Boston, MA.

Alexander Rotenberg (A)

Department of Neurology, Boston Children's Hospital, Harvard Medical School, Boston, MA.

Matthias A Hediger (MA)

Department of Nephrology and Hypertension, University Hospital Bern, Inselspital, Bern, Switzerland.
Institute of Biochemistry and Molecular Medicine, University of Bern, Bern, Switzerland.

Lance H Rodan (LH)

Division of Genetics and Genomics, Boston Children's Hospital, Harvard Medical School, Boston, MA.
Department of Neurology, Boston Children's Hospital, Harvard Medical School, Boston, MA.

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