Expression of the cold thermoreceptor TRPM8 in rodent brain thermoregulatory circuits.
RRID:AB_10989474
RRID:AB_141359
RRID:AB_2079751
RRID:AB_2340375
RRID:AB_2631173
RRID:AB_2721225
RRID:AB_300798
RRID:AB_477652
RRID:AB_514497
RRID:AB_514500
TRPM8
hypothalamus
limbic system
reticular thalamus
rodents
septum
Journal
The Journal of comparative neurology
ISSN: 1096-9861
Titre abrégé: J Comp Neurol
Pays: United States
ID NLM: 0406041
Informations de publication
Date de publication:
01 2021
01 2021
Historique:
received:
05
11
2018
revised:
26
03
2019
accepted:
26
03
2019
pubmed:
4
4
2019
medline:
17
12
2021
entrez:
4
4
2019
Statut:
ppublish
Résumé
The cold- and menthol-activated ion channel transient receptor potential channel subfamily M member 8 (TRPM8) is the principal detector of environmental cold in mammalian sensory nerve endings. Although it is mainly expressed in a subpopulation of peripheral sensory neurons, it has also been identified in non-neuronal tissues. Here, we show, by in situ hybridization (ISH) and by the analysis of transgenic reporter expression in two different reporter mouse strains, that TRPM8 is also expressed in the central nervous system. Although it is present at much lower levels than in peripheral sensory neurons, we found cells expressing TRPM8 in restricted areas of the brain, especially in the hypothalamus, septum, thalamic reticular nucleus, certain cortices and other limbic structures, as well as in some specific nuclei in the brainstem. Interestingly, positive fibers were also found traveling through the major limbic tracts, suggesting a role of TRPM8-expressing central neurons in multiple aspects of thermal regulation, including autonomic and behavioral thermoregulation. Additional ISH experiments in rat brain demonstrated a conserved pattern of expression of this ion channel between rodent species. We confirmed the functional activity of this channel in the mouse brain using electrophysiological patch-clamp recordings of septal neurons. These results open a new window in TRPM8 physiology, guiding further efforts to understand potential roles of this molecular sensor within the brain.
Substances chimiques
TRPM Cation Channels
0
TRPM8 protein, mouse
0
Trpm8 protein, rat
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
234-256Informations de copyright
© 2019 Wiley Periodicals, Inc.
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