Differentiation potential of different regions-derived same donor human Wharton's jelly mesenchymal stem cells into functional smooth muscle-like cells.
Electrophysiology
Mesenchymal stem cells
Smooth muscle cells
Transdifferentiation
Wharton’s jelly
Journal
Cell and tissue research
ISSN: 1432-0878
Titre abrégé: Cell Tissue Res
Pays: Germany
ID NLM: 0417625
Informations de publication
Date de publication:
Aug 2019
Aug 2019
Historique:
received:
06
07
2018
accepted:
19
02
2019
pubmed:
5
4
2019
medline:
18
12
2019
entrez:
5
4
2019
Statut:
ppublish
Résumé
The present study evaluates the transdifferentiation potential of different region-derived same donor Wharton's jelly MSCs (WJMSCs) into functional smooth muscle-like cells (SMLCs). All regions showed baseline expression for early smooth muscle cell (SMC) markers (αSMA and SM22-α) whereas mid marker CALPONIN gradually reduced during in vitro culture expansion and late marker myosin heavy chain type-11 (MHY-11) was completely absent. Furthermore, WJMSCs were induced to SMLCs using DMEM containing 10% FBS supplemented with different concentrations/combinations of TGF-β1 and PDGF-BB under normoxia (20% O
Identifiants
pubmed: 30945004
doi: 10.1007/s00441-019-03009-7
pii: 10.1007/s00441-019-03009-7
doi:
Substances chimiques
Biomarkers
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
229-243Subventions
Organisme : Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (HI13C1596)
ID : NRF-2016R1D1A3B03932491