Multistage continuous countercurrent diafiltration for formulation of monoclonal antibodies.
antibody
continuous processing
countercurrent staging
diafiltration
formulation
Journal
Biotechnology progress
ISSN: 1520-6033
Titre abrégé: Biotechnol Prog
Pays: United States
ID NLM: 8506292
Informations de publication
Date de publication:
07 2019
07 2019
Historique:
received:
25
01
2019
revised:
15
02
2019
accepted:
20
03
2019
pubmed:
6
4
2019
medline:
2
6
2020
entrez:
6
4
2019
Statut:
ppublish
Résumé
There is growing interest in the development of fully integrated and continuous biomanufacturing processes for the production of monoclonal antibody products. A recent study has demonstrated the feasibility of using a two-stage countercurrent diafiltration (DF) process for continuous product formulation, but this system did not provide sufficient levels of buffer exchange for most applications. The objective of this study was to design and test a three-stage countercurrent DF system that could achieve at least 99.9% buffer exchange over 24 hr of continuous operation. Experimental data were obtained using concentrated solutions of human immunoglobulin G as a model protein, with the extent of vitamin B
Substances chimiques
Immunoglobulin G
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2810Informations de copyright
© 2019 American Institute of Chemical Engineers.
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