Alteration of microRNA-4474/4717 expression and CREB-binding protein in human colorectal cancer tissues infected with Fusobacterium nucleatum.
Adult
CREB-Binding Protein
/ genetics
Case-Control Studies
Colorectal Neoplasms
/ genetics
Female
Fusobacterium Infections
/ complications
Fusobacterium nucleatum
/ isolation & purification
Gene Expression Regulation, Neoplastic
Humans
Male
MicroRNAs
/ genetics
Middle Aged
Signal Transduction
Young Adult
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
05
12
2018
accepted:
25
03
2019
entrez:
6
4
2019
pubmed:
6
4
2019
medline:
7
1
2020
Statut:
epublish
Résumé
Colorectal cancer (CRC) is a common and highly lethal form of cancer. Although the etiologic role of Fusobacterium nucleatum (F. nucleatum) in the development of CRC has been elucidated, the specific tumor molecules involved in the progression of CRC induced by F. nucleatum have not been identified. This study investigated several miRNAs and genes involved in the progression of F. nucleatum-induced CRC by Affymetrix miRNA microarray technology and GeneChip Human Transcriptome Array 2.0. The results suggest that miR-4474 and miR-4717 are up-regulated in CRC tissues in response to F. nucleatum infection, compared with the control group (paracancerous tissues), while other genes associated with signaling pathways in cancer, including CREB-binding protein (CREBBP), STAT1, PRKACB, CAMK2B, JUN, TP53 and EWSR1, were dysregulated. Bioinformatic analysis identified CREBBP as the primary aberrantly expressed gene in F. nucleatum-induced CRC. Consistent with the microarray analysis results, real-time RT-PCR analysis demonstrated that the expression of miR-4474/4717 was upregulated while that of CREBBP mRNA was downregulated in CRC patients infected with F. nucleatum. Additionally, CREBBP was identified as a novel target of miR-4474/4717. The results of this study suggest that miR-4474 and miR-4717 are involved in the progression of F. nucleatum-induced CRC by posttranscriptionally regulating the target gene CREBBP.
Identifiants
pubmed: 30951563
doi: 10.1371/journal.pone.0215088
pii: PONE-D-18-34844
pmc: PMC6450631
doi:
Substances chimiques
MIRN4474 microRNA, human
0
MIRN4717 microRNA, human
0
MicroRNAs
0
CREB-Binding Protein
EC 2.3.1.48
CREBBP protein, human
EC 2.3.1.48
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0215088Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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