Molecular typing of Bluetongue virus using the nCounter

Animals Bluetongue virus / classification Capsid Proteins / genetics Cattle / virology DNA Probes / genetics Deer / virology Goats / virology Microarray Analysis / methods Molecular Typing / methods Oligonucleotide Array Sequence Analysis RNA, Viral Serogroup Sheep / virology Viral Load
Bluetongue virus Diagnosis Microarray Nanostring nCounter(®) RNA Typing assays

Journal

Journal of virological methods
ISSN: 1879-0984
Titre abrégé: J Virol Methods
Pays: Netherlands
ID NLM: 8005839

Informations de publication

Date de publication:
07 2019
Historique:
received: 04 02 2019
revised: 31 03 2019
accepted: 01 04 2019
pubmed: 6 4 2019
medline: 4 6 2020
entrez: 6 4 2019
Statut: ppublish

Résumé

Bluetongue virus (BTV) is a segmented double-stranded RNA virus, existing in multiple serotypes, belonging to the genus Orbivirus of the family Reoviridae. BTV causes Bluetongue (BT), a major OIE-listed disease of ruminants. Identification of BTV serotype is accomplished using multiple typing assays and tends to be executed based on the known epidemiological situation within a given country. Samples containing multiple serotypes, particularly those containing novel introductions, may therefore be missed. The aim of this work was to optimize the nCounter® Analysis System Microarray platform (NanoString technologies), that would simultaneously identify all BTV serotypes and co-infections in analyzed samples. Probes were designed according to all Seg-2 sequences, coding for VP2 proteins which determine serotype specificity, available on line. A specific BTV CodeSet of probes was optimized. Experiments were performed with 30 BTV isolates and with 46 field samples previously shown to be infected with BTV by classical molecular assays. All BTV isolates were correctly identified and the expected BTV serotype was recognized in 35 field samples with C

Identifiants

DOI: 10.1016/j.jviromet.2019.04.002 PMID: 30951789
pubmed: 30951789
pii: S0166-0934(19)30046-1
doi: 10.1016/j.jviromet.2019.04.002
pii:
doi:

Substances chimiques

Capsid Proteins 0
DNA Probes 0
RNA, Viral 0
VP2 protein, Bluetongue virus 0

Types de publication

Evaluation Study Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

64-69

Informations de copyright

Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Auteurs

Valentina Curini (V)

OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy; National Reference Center for Whole Genome Sequencing of Microbial Pathogens: Database and Bioinformatic Analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise, Teramo, Italy.

Maurilia Marcacci (M)

OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy; National Reference Center for Whole Genome Sequencing of Microbial Pathogens: Database and Bioinformatic Analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise, Teramo, Italy.

Alfreda Tonelli (A)

OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy; National Reference Center for Whole Genome Sequencing of Microbial Pathogens: Database and Bioinformatic Analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise, Teramo, Italy.

Giovanni Di Teodoro (G)

OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy; National Reference Center for Whole Genome Sequencing of Microbial Pathogens: Database and Bioinformatic Analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise, Teramo, Italy.

Marco Di Domenico (M)

OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy; National Reference Center for Whole Genome Sequencing of Microbial Pathogens: Database and Bioinformatic Analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise, Teramo, Italy.

Nicola D'Alterio (N)

OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy; National Reference Center for Whole Genome Sequencing of Microbial Pathogens: Database and Bioinformatic Analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise, Teramo, Italy.

Ottavio Portanti (O)

OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy; National Reference Center for Whole Genome Sequencing of Microbial Pathogens: Database and Bioinformatic Analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise, Teramo, Italy.

Massimo Ancora (M)

OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy; National Reference Center for Whole Genome Sequencing of Microbial Pathogens: Database and Bioinformatic Analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise, Teramo, Italy.

Giovanni Savini (G)

OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy; National Reference Center for Whole Genome Sequencing of Microbial Pathogens: Database and Bioinformatic Analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise, Teramo, Italy.

Matteo Panfili (M)

Diatech Pharmacogenetics srl, Jesi-Ancona, Italy.

Cesare Camma' (C)

OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy; National Reference Center for Whole Genome Sequencing of Microbial Pathogens: Database and Bioinformatic Analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise, Teramo, Italy.

Alessio Lorusso (A)

OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy; National Reference Center for Whole Genome Sequencing of Microbial Pathogens: Database and Bioinformatic Analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise, Teramo, Italy. Electronic address: a.lorusso@izs.it.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Molecular typing of Bluetongue virus using the nCounter
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN double brin › Reoviridae › Orbivirus › Virus de la langue bleue › Molecular typing of Bluetongue virus using the nCounter
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Protéines virales structurales › Protéines nucléocapside › Protéines de capside › Molecular typing of Bluetongue virus using the nCounter
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Ruminants › Bovins › Molecular typing of Bluetongue virus using the nCounter
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Sondes moléculaires › Sondes d'acide nucléique › Sondes d'ADN › Molecular typing of Bluetongue virus using the nCounter
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Ruminants › Cervidae › Molecular typing of Bluetongue virus using the nCounter
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Ruminants › Capra › Molecular typing of Bluetongue virus using the nCounter
questionsmedicales.fr › Techniques d'investigation › Procédures d'analyse sur micropuce › Analyse sur microréseau › Molecular typing of Bluetongue virus using the nCounter
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Typage moléculaire › Molecular typing of Bluetongue virus using the nCounter
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Séquençage par oligonucléotides en batterie › Molecular typing of Bluetongue virus using the nCounter
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN viral › Molecular typing of Bluetongue virus using the nCounter
questionsmedicales.fr › Phénomènes génétiques › Phénotype › Sérogroupe › Molecular typing of Bluetongue virus using the nCounter
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Ruminants › Ovis › Molecular typing of Bluetongue virus using the nCounter
questionsmedicales.fr › Phénomènes microbiologiques › Phénomènes physiologiques viraux › Charge virale › Molecular typing of Bluetongue virus using the nCounter