The simultaneous downregulation of TRPM7 and MagT1 in human mesenchymal stem cells in vitro: Effects on growth and osteogenic differentiation.
Autophagy
MagT1
Mesenchymal stem cells
Osteogenic differentiation
TRPM7
Journal
Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516
Informations de publication
Date de publication:
21 05 2019
21 05 2019
Historique:
received:
18
03
2019
accepted:
26
03
2019
pubmed:
7
4
2019
medline:
9
4
2020
entrez:
7
4
2019
Statut:
ppublish
Résumé
The magnesium transporters TRPM7 and MagT1 are overexpressed in osteoblastogenesis. We have shown that silencing either TRPM7 or MagT1 accelerates the osteogenic differentiation of human bone mesenchymal stem cells. Here we demonstrate that the simultaneous downregulation of TRPM7 and MagT1 inhibits cell growth and activates autophagy, which is required in the early phases of osteoblastogenesis. In TRPM7/MagT1 downregulating cells the expression of two transcription factors required for activating osteogenesis, i.e. RUNX2 and OSTERIX, is induced more than in the controls both in the presence and in the absence of osteogenic stimuli, while COL1A1 is upregulated in co-silencing cells as much as in the controls. This explains why we found no differences in calcium deposition. We conclude that one of the two transporters should be expressed to accelerate osteogenic differentiation.
Identifiants
pubmed: 30952425
pii: S0006-291X(19)30579-0
doi: 10.1016/j.bbrc.2019.03.178
pii:
doi:
Substances chimiques
Cation Transport Proteins
0
MagT1 protein, human
0
TRPM Cation Channels
0
Protein Serine-Threonine Kinases
EC 2.7.11.1
TRPM7 protein, human
EC 2.7.11.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
159-165Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.