Induction of CD3+ and FoxP3+ T Cells in Left-sided Colorectal Tumors After UFT/LV Chemotherapy.
Adult
Aged
Aged, 80 and over
Antigens, CD
/ genetics
Antineoplastic Combined Chemotherapy Protocols
/ administration & dosage
CD3 Complex
/ immunology
CTLA-4 Antigen
/ genetics
Colorectal Neoplasms
/ drug therapy
Female
Forkhead Transcription Factors
/ immunology
Gene Expression Regulation, Neoplastic
/ drug effects
Humans
Leucovorin
/ administration & dosage
Lymphocytes, Tumor-Infiltrating
/ drug effects
Male
Middle Aged
T-Lymphocytes
/ drug effects
Tegafur
/ administration & dosage
Uracil
/ administration & dosage
Lymphocyte Activation Gene 3 Protein
CD3+ T cells
Colorectal cancer
FoxP3+ regulatory T cells
tumor-infiltrating lymphocytes (TILs)
uracil-tegafur plus leucovorin (UFT/LV) chemotherapy
Journal
Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988
Informations de publication
Date de publication:
Apr 2019
Apr 2019
Historique:
received:
03
03
2019
revised:
17
03
2019
accepted:
18
03
2019
entrez:
7
4
2019
pubmed:
7
4
2019
medline:
16
4
2019
Statut:
ppublish
Résumé
Immune checkpoint inhibitors are mainly used for right-sided, microsatellite instability-high colorectal tumors. In this study, the effects of oral uracil-tegafur plus leucovorin (UFT/LV) chemotherapy on the gene expressions of four immunotherapy targets and the amounts of tumor-infiltrating lymphocytes (TILs) were investigated. Data of 260 patients with stage II or stage III colorectal cancer were analyzed. Gene expression and amount of TILs were evaluated using real-time reverse transcription polymerase chain reaction (CRT-PCR) assay and immunohistochemical staining, respectively. Expression of CTLA4 and LAG3 in tumor tissues was significantly increased after UFT/LV chemotherapy, but only in left-sided tumors. The percentage of high-TIL, high-CD3 and high-FoxP3 patients in the UFT/LV group was significantly higher than that in the control group, only in left-sided tumors. The increase in TILs count, especially of CD3+ T cells and FoxP3+ regulatory T cells, after UFT/LV chemotherapy were specific to left-sided colorectal cancers.
Sections du résumé
BACKGROUND/AIM
OBJECTIVE
Immune checkpoint inhibitors are mainly used for right-sided, microsatellite instability-high colorectal tumors. In this study, the effects of oral uracil-tegafur plus leucovorin (UFT/LV) chemotherapy on the gene expressions of four immunotherapy targets and the amounts of tumor-infiltrating lymphocytes (TILs) were investigated.
PATIENTS AND METHODS
METHODS
Data of 260 patients with stage II or stage III colorectal cancer were analyzed. Gene expression and amount of TILs were evaluated using real-time reverse transcription polymerase chain reaction (CRT-PCR) assay and immunohistochemical staining, respectively.
RESULTS
RESULTS
Expression of CTLA4 and LAG3 in tumor tissues was significantly increased after UFT/LV chemotherapy, but only in left-sided tumors. The percentage of high-TIL, high-CD3 and high-FoxP3 patients in the UFT/LV group was significantly higher than that in the control group, only in left-sided tumors.
CONCLUSION
CONCLUSIONS
The increase in TILs count, especially of CD3+ T cells and FoxP3+ regulatory T cells, after UFT/LV chemotherapy were specific to left-sided colorectal cancers.
Identifiants
pubmed: 30952743
pii: 39/4/1997
doi: 10.21873/anticanres.13310
doi:
Substances chimiques
Antigens, CD
0
CD3 Complex
0
CTLA-4 Antigen
0
CTLA4 protein, human
0
FOXP3 protein, human
0
Forkhead Transcription Factors
0
Tegafur
1548R74NSZ
Uracil
56HH86ZVCT
Leucovorin
Q573I9DVLP
Lymphocyte Activation Gene 3 Protein
0
Lag3 protein, human
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1997-2005Informations de copyright
Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.