ATG2 transports lipids to promote autophagosome biogenesis.
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
03 06 2019
03 06 2019
Historique:
received:
26
11
2018
revised:
05
03
2019
accepted:
28
03
2019
pubmed:
7
4
2019
medline:
10
4
2020
entrez:
7
4
2019
Statut:
ppublish
Résumé
During macroautophagic stress, autophagosomes can be produced continuously and in high numbers. Many different organelles have been reported as potential donor membranes for this sustained autophagosome growth, but specific machinery to support the delivery of lipid to the growing autophagosome membrane has remained unknown. Here we show that the autophagy protein, ATG2, without a clear function since its discovery over 20 yr ago, is in fact a lipid-transfer protein likely operating at the ER-autophagosome interface. ATG2A can bind tens of glycerophospholipids at once and transfers lipids robustly in vitro. An N-terminal fragment of ATG2A that supports lipid transfer in vitro is both necessary and fully sufficient to rescue blocked autophagosome biogenesis in
Identifiants
pubmed: 30952800
pii: jcb.201811139
doi: 10.1083/jcb.201811139
pmc: PMC6548141
doi:
Substances chimiques
ATG2A protein, human
0
ATG2B protein, human
0
Autophagy-Related Proteins
0
Lipids
0
Vesicular Transport Proteins
0
Banques de données
PDB
['6CBC']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1787-1798Subventions
Organisme : NIGMS NIH HHS
ID : T32 GM007223
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM114068
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS063973
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM131715
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM080616
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2019 Valverde et al.
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