Safer Vitrification of Mouse and Human Embryos Using the Novel Cryoroom Vitrification System for Assisted Reproductive Technology.
Journal
Cryo letters
ISSN: 0143-2044
Titre abrégé: Cryo Letters
Pays: England
ID NLM: 9891832
Informations de publication
Date de publication:
Historique:
entrez:
8
4
2019
pubmed:
8
4
2019
medline:
3
8
2019
Statut:
ppublish
Résumé
Vitrification is widely used for assisted reproductive technology (ART). Most vitrification devices require the skillful placement of embryos into the carrier and aspiration of excessive vitrification solution. To evaluate the efficacy and safety of the Cryoroom as a vitrification device. Mouse and human embryos were vitrified with Cryoroom or Cryotop, and the developmental potency was assessed in vitro. Mouse monozygotic twin blastocysts were vitrified with Cryoroom or Cryotop for microarray analysis. In mouse and human embryos, there were no differences between the survival and developmental progress in each device. In silico, the Cryoroom device showed no changes, particularly in DNA methylation after vitrification compared with the Cryotop. These results showed that the form and function of the device may affect the gene expression levels in vitrified embryos. The Cryoroom represents a safe and potentially revolutionary vitrification device for ART.
Sections du résumé
BACKGROUND
BACKGROUND
Vitrification is widely used for assisted reproductive technology (ART). Most vitrification devices require the skillful placement of embryos into the carrier and aspiration of excessive vitrification solution.
OBJECTIVE
OBJECTIVE
To evaluate the efficacy and safety of the Cryoroom as a vitrification device.
MATERIALS AND METHODS
METHODS
Mouse and human embryos were vitrified with Cryoroom or Cryotop, and the developmental potency was assessed in vitro. Mouse monozygotic twin blastocysts were vitrified with Cryoroom or Cryotop for microarray analysis.
RESULTS AND DISCUSSION
CONCLUSIONS
In mouse and human embryos, there were no differences between the survival and developmental progress in each device. In silico, the Cryoroom device showed no changes, particularly in DNA methylation after vitrification compared with the Cryotop. These results showed that the form and function of the device may affect the gene expression levels in vitrified embryos.
CONCLUSION
CONCLUSIONS
The Cryoroom represents a safe and potentially revolutionary vitrification device for ART.
Types de publication
Journal Article
Langues
eng