Time-dependent Effects of Pressure during Preservation of Rat Hearts in an Isochoric System at Subfreezing Temperatures.
Journal
Cryo letters
ISSN: 0143-2044
Titre abrégé: Cryo Letters
Pays: England
ID NLM: 9891832
Informations de publication
Date de publication:
Historique:
entrez:
8
4
2019
pubmed:
8
4
2019
medline:
3
8
2019
Statut:
ppublish
Résumé
Isochoric freezing systems enable ice-free preservation of biological matter at subfreezing temperatures under the increased hydrostatic pressure. To examine the effects of pressure and exposure period on rat hearts preserved in an isochoric chamber. Rat hearts were preserved in the UW solution in isochoric chambers at temperatures from -2°C to -8°C and pressure from the atmospheric level to 78 MPa for up to eight hours, with and without the addition of glycerol. Hearts were evaluated via Langendorff perfusion and HE histology. Hearts were compromised quickly as pressure increased, suggesting an acute time-pressure sensitivity. With the addition of 1 M glycerol, which reduces the pressure experienced at a given temperature, the survival time at -4°C was doubled. The enhanced hydrostatic pressure encountered during isochoric preservation yields time-dependent negative effects on the heart, which can potentially be alleviated by the addition of a cryoprotectant.
Sections du résumé
BACKGROUND
BACKGROUND
Isochoric freezing systems enable ice-free preservation of biological matter at subfreezing temperatures under the increased hydrostatic pressure.
OBJECTIVE
OBJECTIVE
To examine the effects of pressure and exposure period on rat hearts preserved in an isochoric chamber.
MATERIALS AND METHODS
METHODS
Rat hearts were preserved in the UW solution in isochoric chambers at temperatures from -2°C to -8°C and pressure from the atmospheric level to 78 MPa for up to eight hours, with and without the addition of glycerol. Hearts were evaluated via Langendorff perfusion and HE histology.
RESULTS
RESULTS
Hearts were compromised quickly as pressure increased, suggesting an acute time-pressure sensitivity. With the addition of 1 M glycerol, which reduces the pressure experienced at a given temperature, the survival time at -4°C was doubled.
CONCLUSION
CONCLUSIONS
The enhanced hydrostatic pressure encountered during isochoric preservation yields time-dependent negative effects on the heart, which can potentially be alleviated by the addition of a cryoprotectant.
Substances chimiques
Insulin
0
Isochores
0
Organ Preservation Solutions
0
University of Wisconsin-lactobionate solution
0
Allopurinol
63CZ7GJN5I
Glutathione
GAN16C9B8O
Adenosine
K72T3FS567
Raffinose
N5O3QU595M
Types de publication
Journal Article
Langues
eng